Cadmium accumulation and DNA homology with metal resistance genes in sulfate-reducing bacteria

Naghma Naz (Lead / Corresponding author), Hilary K. Young, Nuzhat Ahmed, Geoffrey M. Gadd

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Cadmium resistance (0.1 to 1.0 mM) was studied in four pure and one mixed culture of sulfate-reducing bacteria (SRB). The growth of the bacteria was monitored with respect to carbon source (lactate) oxidation and sulfate reduction in the presence of various concentrations of cadmium chloride. Two strains Desulfovibrio desulfuricans DSM 1926 and Desulfococcus multivorans DSM 2059 showed the highest resistance to cadmium (0.5 mM). Transmission electron microscopy of the two strains showed intracellular and periplasmic accumulation of cadmium. Dot blot DNA hybridization using the probes for the smtAB, cadAC, and cadD genes indicated the presence of similar genetic determinants of heavy metal resistance in the SRB tested. DNA sequencing of the amplified DNA showed strong nucleotide homology in all the SRB strains with the known smtAB genes encoding synechococcal metallothioneins. Protein homology with the known heavy metal-translocating ATPases was also detected in the cloned amplified DNA of Desulfomicrobium norvegicum I1 and Desulfovibrio desulfuricans DSM 1926, suggesting the presence of multiple genetic mechanisms of metal resistance in the two strains.

Original languageEnglish
Pages (from-to)4610-4618
Number of pages9
JournalApplied and Environmental Microbiology
Volume71
Issue number8
DOIs
Publication statusPublished - Aug 2005

Fingerprint

sulfate-reducing bacteria
metal tolerance
sulfate-reducing bacterium
Cadmium
homology
Sulfates
cadmium
Desulfovibrio desulfuricans
Metals
Bacteria
DNA
gene
metal
Desulfomicrobium norvegicum
Heavy Metals
Genes
genes
heavy metal
Cadmium Chloride
Metallothionein

Cite this

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abstract = "Cadmium resistance (0.1 to 1.0 mM) was studied in four pure and one mixed culture of sulfate-reducing bacteria (SRB). The growth of the bacteria was monitored with respect to carbon source (lactate) oxidation and sulfate reduction in the presence of various concentrations of cadmium chloride. Two strains Desulfovibrio desulfuricans DSM 1926 and Desulfococcus multivorans DSM 2059 showed the highest resistance to cadmium (0.5 mM). Transmission electron microscopy of the two strains showed intracellular and periplasmic accumulation of cadmium. Dot blot DNA hybridization using the probes for the smtAB, cadAC, and cadD genes indicated the presence of similar genetic determinants of heavy metal resistance in the SRB tested. DNA sequencing of the amplified DNA showed strong nucleotide homology in all the SRB strains with the known smtAB genes encoding synechococcal metallothioneins. Protein homology with the known heavy metal-translocating ATPases was also detected in the cloned amplified DNA of Desulfomicrobium norvegicum I1 and Desulfovibrio desulfuricans DSM 1926, suggesting the presence of multiple genetic mechanisms of metal resistance in the two strains.",
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Cadmium accumulation and DNA homology with metal resistance genes in sulfate-reducing bacteria. / Naz, Naghma (Lead / Corresponding author); Young, Hilary K.; Ahmed, Nuzhat; Gadd, Geoffrey M.

In: Applied and Environmental Microbiology, Vol. 71, No. 8, 08.2005, p. 4610-4618.

Research output: Contribution to journalArticle

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