Cajal body dynamics and association with chromatin are ATP-dependent

Melpomeni Platani, Ilya Goldberg, Angus I. Lamond, Jason R. Swedlow

    Research output: Contribution to journalArticle

    205 Citations (Scopus)

    Abstract

    Cajal bodies (CBs) are nuclear organelles that contain factors required for splicing, ribosome biogenesis and transcription. Our previous analysis in living cells showed that CBs are dynamic structures. Here, we show that CB mobility is described by anomalous diffusion and that bodies alternate between association with chromatin and diffusion within the interchromatin space. CB mobility increases after ATP depletion and inhibition of transcription, suggesting that the association of CB and chromatin requires ATP and active transcription. This behaviour is fundamentally different from the ATP-dependent mobility observed for chromatin and suggests that a novel mechanism governs CB, and possibly other, nuclear body dynamics.
    Original languageEnglish
    Pages (from-to)502-508
    Number of pages7
    JournalNature Cell Biology
    Volume4
    Issue number7
    DOIs
    Publication statusPublished - Feb 2002

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    Chromatin
    Adenosine Triphosphate
    Ribosomes
    Organelles
    RNA Splicing Factors

    Cite this

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    title = "Cajal body dynamics and association with chromatin are ATP-dependent",
    abstract = "Cajal bodies (CBs) are nuclear organelles that contain factors required for splicing, ribosome biogenesis and transcription. Our previous analysis in living cells showed that CBs are dynamic structures. Here, we show that CB mobility is described by anomalous diffusion and that bodies alternate between association with chromatin and diffusion within the interchromatin space. CB mobility increases after ATP depletion and inhibition of transcription, suggesting that the association of CB and chromatin requires ATP and active transcription. This behaviour is fundamentally different from the ATP-dependent mobility observed for chromatin and suggests that a novel mechanism governs CB, and possibly other, nuclear body dynamics.",
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    Cajal body dynamics and association with chromatin are ATP-dependent. / Platani, Melpomeni; Goldberg, Ilya; Lamond, Angus I.; Swedlow, Jason R.

    In: Nature Cell Biology, Vol. 4, No. 7, 02.2002, p. 502-508.

    Research output: Contribution to journalArticle

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    T1 - Cajal body dynamics and association with chromatin are ATP-dependent

    AU - Platani, Melpomeni

    AU - Goldberg, Ilya

    AU - Lamond, Angus I.

    AU - Swedlow, Jason R.

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    AB - Cajal bodies (CBs) are nuclear organelles that contain factors required for splicing, ribosome biogenesis and transcription. Our previous analysis in living cells showed that CBs are dynamic structures. Here, we show that CB mobility is described by anomalous diffusion and that bodies alternate between association with chromatin and diffusion within the interchromatin space. CB mobility increases after ATP depletion and inhibition of transcription, suggesting that the association of CB and chromatin requires ATP and active transcription. This behaviour is fundamentally different from the ATP-dependent mobility observed for chromatin and suggests that a novel mechanism governs CB, and possibly other, nuclear body dynamics.

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