Cell movement during chick primitive streak formation

Manli Chuai, Wei Zeng, Xuesong Yang, Veronika Boychenko, James A. Glazier, Cornelis J. Weijer

    Research output: Contribution to journalArticle

    81 Citations (Scopus)

    Abstract

    Gastrulation in amniotes begins with extensive re-arrangements of cells in the epiblast resulting in the formation of the primitive streak. We have developed a transfection method that enables us to transfect randomly distributed epiblast cells in the Stage XI–XIII chick blastoderms with GFP fusion proteins. This allows us to use time-lapse microscopy for detailed analysis of the movements and proliferation of epiblast cells during streak formation. Cells in the posterior two thirds of the embryo move in two striking counter-rotating flows that meet at the site of streak formation at the posterior end of the embryo. Cells divide during this rotational movement with a cell cycle time of 6–7 h. Daughter cells remain together, forming small clusters and as result of the flow patterns line up in the streak. Expression of the cyclin-dependent kinase inhibitor, P21/Waf inhibits cell division and severely limits embryo growth, but does not inhibit streak formation or associated flows. To investigate the role off cell–cell intercalation in streak formation we have inhibited the Wnt planar-polarity signalling pathway by expression of a dominant negative Wnt11 and a Dishevelled mutant Xdd1. Both treatments do not result in an inhibition of streak formation, but both severely affect extension of the embryo in later development. Likewise inhibition of myosin II which as been shown to drive cell–cell intercalation during Drosophila germ band extension, has no effect on streak formation, but also effectively blocks elongation after regression has started. These experiments make it unlikely that streak formation involves known cell–cell intercalation mechanisms. Expression of a dominant negative FGFR1c receptor construct as well as the soluble extracellular domain of the FGFR1c receptor both effectively block the cell movements associated with streak formation and mesoderm differentiation, showing the importance of FGF signalling in these processes.
    Original languageEnglish
    Pages (from-to)137-149
    Number of pages13
    JournalDevelopmental Biology
    Volume296
    Issue number1
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    Primitive Streak
    Cell Movement
    Germ Layers
    Embryonic Structures
    Blastoderm
    Cyclin-Dependent Kinase Inhibitor p21
    Myosin Type II
    Gastrulation
    Mesoderm
    Cell Division
    Drosophila
    Transfection
    Microscopy
    Cell Cycle
    Cell Proliferation
    Growth
    Proteins

    Keywords

    • Primitive streak
    • Cell division
    • Cell intercalation
    • FGF signalling

    Cite this

    Chuai, Manli ; Zeng, Wei ; Yang, Xuesong ; Boychenko, Veronika ; Glazier, James A. ; Weijer, Cornelis J. / Cell movement during chick primitive streak formation. In: Developmental Biology. 2006 ; Vol. 296, No. 1. pp. 137-149.
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    Cell movement during chick primitive streak formation. / Chuai, Manli; Zeng, Wei; Yang, Xuesong; Boychenko, Veronika; Glazier, James A.; Weijer, Cornelis J.

    In: Developmental Biology, Vol. 296, No. 1, 2006, p. 137-149.

    Research output: Contribution to journalArticle

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    AU - Chuai, Manli

    AU - Zeng, Wei

    AU - Yang, Xuesong

    AU - Boychenko, Veronika

    AU - Glazier, James A.

    AU - Weijer, Cornelis J.

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    KW - Cell division

    KW - Cell intercalation

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