Characterization of the cross-reacting determinant (crd) of the glycosyl-phosphatidylinositol membrane anchor of trypanosoma-brucei variant surface glycoprotein

Susanne E. Zamze, Michael A. J. Ferguson, Ruth Collins, Raymond A. Dwek, Thomas W. Rademacher

    Research output: Contribution to journalArticle

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    Abstract

    The cross-reacting determinant (CRD epitope) of the glycosyl-phosphatidylinositol (GPI) membrane anchor of Tryponosoma brucei glycoprotein has been analysed by selective chemical and enzymic modification of the isolated GPI structure combined with the use of a competitive ELISA inhibition assay for the detection of CRD epitopes. The data show that the CRD consists of at least three overlapping epitopes involving different regions of the molecule including the inositol 1, 2-cyclic phosphate, the non-N-acetylated-glucosamine residue and the galactose branch. Although the presence of all three of these structural features is required for quantitative binding of anti-CRD antibodies in ELISA and Western blotting, the Western blot reaction obtained inthe presence of any one epitope is still significant. The use of anti-CRD antibodies for the detection of GPI anchors is discussed.

    Original languageEnglish
    Pages (from-to)527-534
    Number of pages8
    JournalEuropean Journal of Biochemistry
    Volume176
    Issue number3
    DOIs
    Publication statusPublished - Oct 1988

    Cite this

    @article{2909dabfaefe45b0be2acb9d2f130f37,
    title = "Characterization of the cross-reacting determinant (crd) of the glycosyl-phosphatidylinositol membrane anchor of trypanosoma-brucei variant surface glycoprotein",
    abstract = "The cross-reacting determinant (CRD epitope) of the glycosyl-phosphatidylinositol (GPI) membrane anchor of Tryponosoma brucei glycoprotein has been analysed by selective chemical and enzymic modification of the isolated GPI structure combined with the use of a competitive ELISA inhibition assay for the detection of CRD epitopes. The data show that the CRD consists of at least three overlapping epitopes involving different regions of the molecule including the inositol 1, 2-cyclic phosphate, the non-N-acetylated-glucosamine residue and the galactose branch. Although the presence of all three of these structural features is required for quantitative binding of anti-CRD antibodies in ELISA and Western blotting, the Western blot reaction obtained inthe presence of any one epitope is still significant. The use of anti-CRD antibodies for the detection of GPI anchors is discussed.",
    author = "Zamze, {Susanne E.} and Ferguson, {Michael A. J.} and Ruth Collins and Dwek, {Raymond A.} and Rademacher, {Thomas W.}",
    year = "1988",
    month = "10",
    doi = "10.1111/j.1432-1033.1988.tb14310.x",
    language = "English",
    volume = "176",
    pages = "527--534",
    journal = "European Journal of Biochemistry",
    issn = "0014-2956",
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    Characterization of the cross-reacting determinant (crd) of the glycosyl-phosphatidylinositol membrane anchor of trypanosoma-brucei variant surface glycoprotein. / Zamze, Susanne E.; Ferguson, Michael A. J.; Collins, Ruth; Dwek, Raymond A.; Rademacher, Thomas W.

    In: European Journal of Biochemistry, Vol. 176, No. 3, 10.1988, p. 527-534.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Characterization of the cross-reacting determinant (crd) of the glycosyl-phosphatidylinositol membrane anchor of trypanosoma-brucei variant surface glycoprotein

    AU - Zamze, Susanne E.

    AU - Ferguson, Michael A. J.

    AU - Collins, Ruth

    AU - Dwek, Raymond A.

    AU - Rademacher, Thomas W.

    PY - 1988/10

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    AB - The cross-reacting determinant (CRD epitope) of the glycosyl-phosphatidylinositol (GPI) membrane anchor of Tryponosoma brucei glycoprotein has been analysed by selective chemical and enzymic modification of the isolated GPI structure combined with the use of a competitive ELISA inhibition assay for the detection of CRD epitopes. The data show that the CRD consists of at least three overlapping epitopes involving different regions of the molecule including the inositol 1, 2-cyclic phosphate, the non-N-acetylated-glucosamine residue and the galactose branch. Although the presence of all three of these structural features is required for quantitative binding of anti-CRD antibodies in ELISA and Western blotting, the Western blot reaction obtained inthe presence of any one epitope is still significant. The use of anti-CRD antibodies for the detection of GPI anchors is discussed.

    U2 - 10.1111/j.1432-1033.1988.tb14310.x

    DO - 10.1111/j.1432-1033.1988.tb14310.x

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    JF - European Journal of Biochemistry

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