Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore

L. J. Bock, C. Pagliuca, C. Alfieri, C. Golfieri, M. Dal Maschio, P. De Wulf, N. Kobayashi, Y. Oku, T.U. Tanaka, R.A. Grove, K. Shrestha, T.R. Hazbun, A. Oldani, R. Bermejo

    Research output: Contribution to journalArticle

    57 Citations (Scopus)

    Abstract

    Kinetochores attach the replicated chromosomes to the mitotic spindle and orchestrate their transmission to the daughter cells. Kinetochore-spindle binding and chromosome segregation are mediated by the multi-copy KNL1 , MIS12 and NDC80 complexes that form the so-called KMN network. KMN-spindle attachment is regulated by the AuroraB and MPS1 kinases. It is unclear whether other mechanisms exist that support KMN activity during the cell cycle. Using budding yeast, we show that kinetochore protein Cnn1 localizes to the base of the Ndc80 complex and promotes a functionally competent configuration of the KMN network. Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes. Cnn1 activity peaks in anaphase and is driven by the Cdc28, Mps1 and Ipl1 kinases.
    Original languageEnglish
    Pages (from-to)614-624
    Number of pages11
    JournalNature Cell Biology
    Volume14
    Issue number6
    DOIs
    Publication statusPublished - 2012

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    Kinetochores
    Yeasts
    Phosphotransferases
    Chromosome Segregation
    Anaphase
    Spindle Apparatus
    Saccharomycetales
    Cell Cycle
    Chromosomes
    Proteins

    Cite this

    Bock, L. J., Pagliuca, C., Alfieri, C., Golfieri, C., Dal Maschio, M., De Wulf, P., ... Bermejo, R. (2012). Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore. Nature Cell Biology, 14(6), 614-624. https://doi.org/10.1038/ncb2495
    Bock, L. J. ; Pagliuca, C. ; Alfieri, C. ; Golfieri, C. ; Dal Maschio, M. ; De Wulf, P. ; Kobayashi, N. ; Oku, Y. ; Tanaka, T.U. ; Grove, R.A. ; Shrestha, K. ; Hazbun, T.R. ; Oldani, A. ; Bermejo, R. / Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore. In: Nature Cell Biology. 2012 ; Vol. 14, No. 6. pp. 614-624.
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    abstract = "Kinetochores attach the replicated chromosomes to the mitotic spindle and orchestrate their transmission to the daughter cells. Kinetochore-spindle binding and chromosome segregation are mediated by the multi-copy KNL1 , MIS12 and NDC80 complexes that form the so-called KMN network. KMN-spindle attachment is regulated by the AuroraB and MPS1 kinases. It is unclear whether other mechanisms exist that support KMN activity during the cell cycle. Using budding yeast, we show that kinetochore protein Cnn1 localizes to the base of the Ndc80 complex and promotes a functionally competent configuration of the KMN network. Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes. Cnn1 activity peaks in anaphase and is driven by the Cdc28, Mps1 and Ipl1 kinases.",
    author = "Bock, {L. J.} and C. Pagliuca and C. Alfieri and C. Golfieri and {Dal Maschio}, M. and {De Wulf}, P. and N. Kobayashi and Y. Oku and T.U. Tanaka and R.A. Grove and K. Shrestha and T.R. Hazbun and A. Oldani and R. Bermejo",
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    Bock, LJ, Pagliuca, C, Alfieri, C, Golfieri, C, Dal Maschio, M, De Wulf, P, Kobayashi, N, Oku, Y, Tanaka, TU, Grove, RA, Shrestha, K, Hazbun, TR, Oldani, A & Bermejo, R 2012, 'Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore', Nature Cell Biology, vol. 14, no. 6, pp. 614-624. https://doi.org/10.1038/ncb2495

    Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore. / Bock, L. J.; Pagliuca, C.; Alfieri, C.; Golfieri, C.; Dal Maschio, M.; De Wulf, P.; Kobayashi, N.; Oku, Y.; Tanaka, T.U.; Grove, R.A.; Shrestha, K.; Hazbun, T.R.; Oldani, A.; Bermejo, R.

    In: Nature Cell Biology, Vol. 14, No. 6, 2012, p. 614-624.

    Research output: Contribution to journalArticle

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    AU - Bock, L. J.

    AU - Pagliuca, C.

    AU - Alfieri, C.

    AU - Golfieri, C.

    AU - Dal Maschio, M.

    AU - De Wulf, P.

    AU - Kobayashi, N.

    AU - Oku, Y.

    AU - Tanaka, T.U.

    AU - Grove, R.A.

    AU - Shrestha, K.

    AU - Hazbun, T.R.

    AU - Oldani, A.

    AU - Bermejo, R.

    N1 - Copyright 2012 Elsevier B.V., All rights reserved.

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    AB - Kinetochores attach the replicated chromosomes to the mitotic spindle and orchestrate their transmission to the daughter cells. Kinetochore-spindle binding and chromosome segregation are mediated by the multi-copy KNL1 , MIS12 and NDC80 complexes that form the so-called KMN network. KMN-spindle attachment is regulated by the AuroraB and MPS1 kinases. It is unclear whether other mechanisms exist that support KMN activity during the cell cycle. Using budding yeast, we show that kinetochore protein Cnn1 localizes to the base of the Ndc80 complex and promotes a functionally competent configuration of the KMN network. Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes. Cnn1 activity peaks in anaphase and is driven by the Cdc28, Mps1 and Ipl1 kinases.

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    Bock LJ, Pagliuca C, Alfieri C, Golfieri C, Dal Maschio M, De Wulf P et al. Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore. Nature Cell Biology. 2012;14(6):614-624. https://doi.org/10.1038/ncb2495