Direct non transcriptional role of NF-Y in DNA replication

Paolo Benatti, Silvia Belluti, Benoit Miotto, Julia Neusiedler, Diletta Dolfini, Marjorie Drac, Valentina Basile, Etienne Schwob, Roberto Mantovani, J. Julian Blow, Carol Imbriano (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

NF-Y is a heterotrimeric transcription factor, which plays a pioneer role in the transcriptional control of promoters containing the CCAAT-box, among which genes involved in cell cycle regulation, apoptosis and DNA damage response. The knock-down of the sequence-specific subunit NF-YA triggers defects in S-phase progression, which lead to apoptotic cell death. Here, we report that NF-Y has a critical function in DNA replication progression, independent from its transcriptional activity. NF-YA colocalizes with early DNA replication factories, its depletion affects the loading of replisome proteins to DNA, among which Cdc45, and delays the passage from early to middle-late S phase. Molecular combing experiments are consistent with a role for NF-Y in the control of fork progression. Finally, we unambiguously demonstrate a direct non-transcriptional role of NF-Y in the overall efficiency of DNA replication, specifically in the DNA elongation process, using a Xenopus cell-free system. Our findings broaden the activity of NF-Y on a DNA metabolism other than transcription, supporting the existence of specific TFs required for proper and efficient DNA replication.

Original languageEnglish
Pages (from-to)673-685
Number of pages13
JournalBiochimica et Biophysica Acta. Molecular Cell Research
Volume1863
Issue number4
Early online date28 Dec 2015
DOIs
Publication statusPublished - 1 Apr 2016

Fingerprint

DNA Replication
S Phase
DNA
Cell-Free System
Xenopus
DNA Damage
Cell Cycle
Cell Death
Transcription Factors
Apoptosis
Genes
Proteins

Keywords

  • CCAAT-binding factor
  • DNA replication
  • NF-Y
  • Transcription factors
  • Xenopus cell-free system

Cite this

Benatti, P., Belluti, S., Miotto, B., Neusiedler, J., Dolfini, D., Drac, M., ... Imbriano, C. (2016). Direct non transcriptional role of NF-Y in DNA replication. Biochimica et Biophysica Acta. Molecular Cell Research, 1863(4), 673-685. https://doi.org/10.1016/j.bbamcr.2015.12.019
Benatti, Paolo ; Belluti, Silvia ; Miotto, Benoit ; Neusiedler, Julia ; Dolfini, Diletta ; Drac, Marjorie ; Basile, Valentina ; Schwob, Etienne ; Mantovani, Roberto ; Blow, J. Julian ; Imbriano, Carol. / Direct non transcriptional role of NF-Y in DNA replication. In: Biochimica et Biophysica Acta. Molecular Cell Research. 2016 ; Vol. 1863, No. 4. pp. 673-685.
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Benatti, P, Belluti, S, Miotto, B, Neusiedler, J, Dolfini, D, Drac, M, Basile, V, Schwob, E, Mantovani, R, Blow, JJ & Imbriano, C 2016, 'Direct non transcriptional role of NF-Y in DNA replication', Biochimica et Biophysica Acta. Molecular Cell Research, vol. 1863, no. 4, pp. 673-685. https://doi.org/10.1016/j.bbamcr.2015.12.019

Direct non transcriptional role of NF-Y in DNA replication. / Benatti, Paolo; Belluti, Silvia; Miotto, Benoit; Neusiedler, Julia; Dolfini, Diletta; Drac, Marjorie; Basile, Valentina; Schwob, Etienne; Mantovani, Roberto; Blow, J. Julian; Imbriano, Carol (Lead / Corresponding author).

In: Biochimica et Biophysica Acta. Molecular Cell Research, Vol. 1863, No. 4, 01.04.2016, p. 673-685.

Research output: Contribution to journalArticle

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T1 - Direct non transcriptional role of NF-Y in DNA replication

AU - Benatti, Paolo

AU - Belluti, Silvia

AU - Miotto, Benoit

AU - Neusiedler, Julia

AU - Dolfini, Diletta

AU - Drac, Marjorie

AU - Basile, Valentina

AU - Schwob, Etienne

AU - Mantovani, Roberto

AU - Blow, J. Julian

AU - Imbriano, Carol

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