Exo70, a subunit of the exocyst complex, interacts with SNEVhPrp19/hPso4 and is involved in pre-mRNA splicing

Hanna Dellago, Marlies Löscher , Paul Ajuh, Ursula Ryder, Christian Kaisermayer, Regina Grillari-Voglauer, Klaus Fortschegger, Stefan Gross, Anna Gstraunthaler, Nicole Borth, Frank Eisenhaber, Angus I. Lamond, Johannes Grillari

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    The Cdc5L (cell division cycle 5-like) complex is a spliceosomal subcomplex that also plays a role in DNA repair. The complex contains the splicing factor hPrp19, also known as SNEV or hPso4, which is involved in cellular life-span regulation and proteasomal breakdown. In a recent large-scale proteomics analysis for proteins associated with this complex, proteins involved in transcription, cell-cycle regulation, DNA repair, the ubiquitin-proteasome system, chromatin remodelling, cellular aging, the cytoskeleton and trafficking, including four members of the exocyst complex, were identified. In the present paper we report that Exo70 interacts directly with SNEVhPrp19/hPso4 and shuttles to the nucleus, where it associates with the spliceosome. We mapped the interaction site to the N-terminal 100 amino acids of Exo70, which interfere with pre-mRNA splicing in vitro. Furthermore, Exo70 influences the splicing of a model substrate as well as of its own pre-mRNA in vivo. In addition, we found that Exo70 is alternatively spliced in a cell-type- and cell-age-dependent way. These results suggest a novel and unexpected role of Exo70 in nuclear mRNA splicing, where it might signal membrane events to the splicing apparatus.

    Original languageEnglish
    Pages (from-to)81-91
    Number of pages11
    JournalBiochemical Journal
    Volume438
    DOIs
    Publication statusPublished - 15 Aug 2011

    Keywords

    • alternative splicing
    • exocyst
    • Exo70
    • protein interaction
    • Prp19
    • SNEV
    • NUCLEAR EXPORT SIGNALS
    • DIPLOID CELL STRAINS
    • ENDOTHELIAL-CELLS
    • MASS-SPECTROMETRY
    • SUBCELLULAR-LOCALIZATION
    • SACCHAROMYCES-CEREVISIAE
    • ENDOPLASMIC-RETICULUM
    • PROTEIN COMPLEXES
    • EPITHELIAL-CELLS
    • SEC6/8 COMPLEX

    Cite this

    Dellago, H., Löscher , M., Ajuh, P., Ryder, U., Kaisermayer, C., Grillari-Voglauer, R., ... Grillari, J. (2011). Exo70, a subunit of the exocyst complex, interacts with SNEVhPrp19/hPso4 and is involved in pre-mRNA splicing. Biochemical Journal, 438, 81-91. https://doi.org/10.1042/BJ20110183
    Dellago, Hanna ; Löscher , Marlies ; Ajuh, Paul ; Ryder, Ursula ; Kaisermayer, Christian ; Grillari-Voglauer, Regina ; Fortschegger, Klaus ; Gross, Stefan ; Gstraunthaler, Anna ; Borth, Nicole ; Eisenhaber, Frank ; Lamond, Angus I. ; Grillari, Johannes. / Exo70, a subunit of the exocyst complex, interacts with SNEVhPrp19/hPso4 and is involved in pre-mRNA splicing. In: Biochemical Journal. 2011 ; Vol. 438. pp. 81-91.
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    abstract = "The Cdc5L (cell division cycle 5-like) complex is a spliceosomal subcomplex that also plays a role in DNA repair. The complex contains the splicing factor hPrp19, also known as SNEV or hPso4, which is involved in cellular life-span regulation and proteasomal breakdown. In a recent large-scale proteomics analysis for proteins associated with this complex, proteins involved in transcription, cell-cycle regulation, DNA repair, the ubiquitin-proteasome system, chromatin remodelling, cellular aging, the cytoskeleton and trafficking, including four members of the exocyst complex, were identified. In the present paper we report that Exo70 interacts directly with SNEVhPrp19/hPso4 and shuttles to the nucleus, where it associates with the spliceosome. We mapped the interaction site to the N-terminal 100 amino acids of Exo70, which interfere with pre-mRNA splicing in vitro. Furthermore, Exo70 influences the splicing of a model substrate as well as of its own pre-mRNA in vivo. In addition, we found that Exo70 is alternatively spliced in a cell-type- and cell-age-dependent way. These results suggest a novel and unexpected role of Exo70 in nuclear mRNA splicing, where it might signal membrane events to the splicing apparatus.",
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    author = "Hanna Dellago and Marlies L{\"o}scher and Paul Ajuh and Ursula Ryder and Christian Kaisermayer and Regina Grillari-Voglauer and Klaus Fortschegger and Stefan Gross and Anna Gstraunthaler and Nicole Borth and Frank Eisenhaber and Lamond, {Angus I.} and Johannes Grillari",
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    Dellago, H, Löscher , M, Ajuh, P, Ryder, U, Kaisermayer, C, Grillari-Voglauer, R, Fortschegger, K, Gross, S, Gstraunthaler, A, Borth, N, Eisenhaber, F, Lamond, AI & Grillari, J 2011, 'Exo70, a subunit of the exocyst complex, interacts with SNEVhPrp19/hPso4 and is involved in pre-mRNA splicing', Biochemical Journal, vol. 438, pp. 81-91. https://doi.org/10.1042/BJ20110183

    Exo70, a subunit of the exocyst complex, interacts with SNEVhPrp19/hPso4 and is involved in pre-mRNA splicing. / Dellago, Hanna; Löscher , Marlies; Ajuh, Paul; Ryder, Ursula; Kaisermayer, Christian; Grillari-Voglauer, Regina; Fortschegger, Klaus; Gross, Stefan; Gstraunthaler, Anna; Borth, Nicole; Eisenhaber, Frank; Lamond, Angus I.; Grillari, Johannes.

    In: Biochemical Journal, Vol. 438, 15.08.2011, p. 81-91.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Exo70, a subunit of the exocyst complex, interacts with SNEVhPrp19/hPso4 and is involved in pre-mRNA splicing

    AU - Dellago, Hanna

    AU - Löscher , Marlies

    AU - Ajuh, Paul

    AU - Ryder, Ursula

    AU - Kaisermayer, Christian

    AU - Grillari-Voglauer, Regina

    AU - Fortschegger, Klaus

    AU - Gross, Stefan

    AU - Gstraunthaler, Anna

    AU - Borth, Nicole

    AU - Eisenhaber, Frank

    AU - Lamond, Angus I.

    AU - Grillari, Johannes

    PY - 2011/8/15

    Y1 - 2011/8/15

    N2 - The Cdc5L (cell division cycle 5-like) complex is a spliceosomal subcomplex that also plays a role in DNA repair. The complex contains the splicing factor hPrp19, also known as SNEV or hPso4, which is involved in cellular life-span regulation and proteasomal breakdown. In a recent large-scale proteomics analysis for proteins associated with this complex, proteins involved in transcription, cell-cycle regulation, DNA repair, the ubiquitin-proteasome system, chromatin remodelling, cellular aging, the cytoskeleton and trafficking, including four members of the exocyst complex, were identified. In the present paper we report that Exo70 interacts directly with SNEVhPrp19/hPso4 and shuttles to the nucleus, where it associates with the spliceosome. We mapped the interaction site to the N-terminal 100 amino acids of Exo70, which interfere with pre-mRNA splicing in vitro. Furthermore, Exo70 influences the splicing of a model substrate as well as of its own pre-mRNA in vivo. In addition, we found that Exo70 is alternatively spliced in a cell-type- and cell-age-dependent way. These results suggest a novel and unexpected role of Exo70 in nuclear mRNA splicing, where it might signal membrane events to the splicing apparatus.

    AB - The Cdc5L (cell division cycle 5-like) complex is a spliceosomal subcomplex that also plays a role in DNA repair. The complex contains the splicing factor hPrp19, also known as SNEV or hPso4, which is involved in cellular life-span regulation and proteasomal breakdown. In a recent large-scale proteomics analysis for proteins associated with this complex, proteins involved in transcription, cell-cycle regulation, DNA repair, the ubiquitin-proteasome system, chromatin remodelling, cellular aging, the cytoskeleton and trafficking, including four members of the exocyst complex, were identified. In the present paper we report that Exo70 interacts directly with SNEVhPrp19/hPso4 and shuttles to the nucleus, where it associates with the spliceosome. We mapped the interaction site to the N-terminal 100 amino acids of Exo70, which interfere with pre-mRNA splicing in vitro. Furthermore, Exo70 influences the splicing of a model substrate as well as of its own pre-mRNA in vivo. In addition, we found that Exo70 is alternatively spliced in a cell-type- and cell-age-dependent way. These results suggest a novel and unexpected role of Exo70 in nuclear mRNA splicing, where it might signal membrane events to the splicing apparatus.

    KW - alternative splicing

    KW - exocyst

    KW - Exo70

    KW - protein interaction

    KW - Prp19

    KW - SNEV

    KW - NUCLEAR EXPORT SIGNALS

    KW - DIPLOID CELL STRAINS

    KW - ENDOTHELIAL-CELLS

    KW - MASS-SPECTROMETRY

    KW - SUBCELLULAR-LOCALIZATION

    KW - SACCHAROMYCES-CEREVISIAE

    KW - ENDOPLASMIC-RETICULUM

    KW - PROTEIN COMPLEXES

    KW - EPITHELIAL-CELLS

    KW - SEC6/8 COMPLEX

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    DO - 10.1042/BJ20110183

    M3 - Article

    VL - 438

    SP - 81

    EP - 91

    JO - Biochemical Journal

    JF - Biochemical Journal

    SN - 0264-6021

    ER -

    Dellago H, Löscher M, Ajuh P, Ryder U, Kaisermayer C, Grillari-Voglauer R et al. Exo70, a subunit of the exocyst complex, interacts with SNEVhPrp19/hPso4 and is involved in pre-mRNA splicing. Biochemical Journal. 2011 Aug 15;438:81-91. https://doi.org/10.1042/BJ20110183