Fluid phase endocytosis within buccal mucosal cells of alcohol misusers

S.E. Axford, G.R. Ogden, A.M. Stewart, H.A. Saleh, P.E. Ross, D. Hopwood

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    The structure of the oral mucosa is now well characterised, although studies on oral epithelial cell function have received less attention. The aims of this study were to see whether endocytosis could be demonstrated in cells from oral smears and, if so, to assess the effect of chronic high alcohol intake on such uptake. Buccal mucosal smears were collected from 135 patients (91 non- or social drinkers, and 44 patients with harmful alcohol use). Name, age, sex, and alcohol history (for alcohol problem patients) were recorded. Cell suspensions were incubated in a solution of bovine serum albumin (BSA)-coated fluorescently labelled latex microspheres (0.02 µm diameter) in Ham's F-10 culture medium for 1 h at 37°C as a marker of fluid phase endocytosis. Uptake of microspheres was confirmed by confocal microscopy, and mean endocytosed fluorescence levels determined by flow cytometry. A repeat smear from 11 of the alcohol patients was taken 9-14 days later. Endocytosis was significantly reduced in both male (P <0.01) and female (P <0.01) alcohol problem patients compared to controls. Units of alcohol consumed and cigarettes smoked per day did not show a dose-response correlation with endocytosis in the alcohol problem patients. Apparent abstinence from alcohol had no further effect on endocytic uptake at days 9- 14. This study shows that normal oral squamous cells removed as buccal smears readily endocytose fluorescent microspheres and that this capacity can be affected by alcohol. Chronic high alcohol intake would appear to down regulate endocytosis in buccal cells even up to 14 days of abstinence. This may have implications for the pathogenesis of oral mucosal disorders in long- term users.
    Original languageEnglish
    Pages (from-to)86-92
    Number of pages7
    JournalOral Oncology
    Volume35
    Issue number1
    DOIs
    Publication statusPublished - 1999

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    Cheek
    Endocytosis
    Alcohols
    Microspheres
    Epithelial Cells
    Alcohol Abstinence
    Latex
    Mouth Mucosa
    Bovine Serum Albumin
    Confocal Microscopy
    Tobacco Products
    Names
    Culture Media
    Suspensions
    Flow Cytometry
    Down-Regulation
    Fluorescence
    History

    Cite this

    Axford, S. E., Ogden, G. R., Stewart, A. M., Saleh, H. A., Ross, P. E., & Hopwood, D. (1999). Fluid phase endocytosis within buccal mucosal cells of alcohol misusers. Oral Oncology, 35(1), 86-92. https://doi.org/10.1016/S1368-8375(98)00086-4
    Axford, S.E. ; Ogden, G.R. ; Stewart, A.M. ; Saleh, H.A. ; Ross, P.E. ; Hopwood, D. / Fluid phase endocytosis within buccal mucosal cells of alcohol misusers. In: Oral Oncology. 1999 ; Vol. 35, No. 1. pp. 86-92.
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    abstract = "The structure of the oral mucosa is now well characterised, although studies on oral epithelial cell function have received less attention. The aims of this study were to see whether endocytosis could be demonstrated in cells from oral smears and, if so, to assess the effect of chronic high alcohol intake on such uptake. Buccal mucosal smears were collected from 135 patients (91 non- or social drinkers, and 44 patients with harmful alcohol use). Name, age, sex, and alcohol history (for alcohol problem patients) were recorded. Cell suspensions were incubated in a solution of bovine serum albumin (BSA)-coated fluorescently labelled latex microspheres (0.02 µm diameter) in Ham's F-10 culture medium for 1 h at 37°C as a marker of fluid phase endocytosis. Uptake of microspheres was confirmed by confocal microscopy, and mean endocytosed fluorescence levels determined by flow cytometry. A repeat smear from 11 of the alcohol patients was taken 9-14 days later. Endocytosis was significantly reduced in both male (P <0.01) and female (P <0.01) alcohol problem patients compared to controls. Units of alcohol consumed and cigarettes smoked per day did not show a dose-response correlation with endocytosis in the alcohol problem patients. Apparent abstinence from alcohol had no further effect on endocytic uptake at days 9- 14. This study shows that normal oral squamous cells removed as buccal smears readily endocytose fluorescent microspheres and that this capacity can be affected by alcohol. Chronic high alcohol intake would appear to down regulate endocytosis in buccal cells even up to 14 days of abstinence. This may have implications for the pathogenesis of oral mucosal disorders in long- term users.",
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    Axford, SE, Ogden, GR, Stewart, AM, Saleh, HA, Ross, PE & Hopwood, D 1999, 'Fluid phase endocytosis within buccal mucosal cells of alcohol misusers', Oral Oncology, vol. 35, no. 1, pp. 86-92. https://doi.org/10.1016/S1368-8375(98)00086-4

    Fluid phase endocytosis within buccal mucosal cells of alcohol misusers. / Axford, S.E.; Ogden, G.R.; Stewart, A.M.; Saleh, H.A.; Ross, P.E.; Hopwood, D.

    In: Oral Oncology, Vol. 35, No. 1, 1999, p. 86-92.

    Research output: Contribution to journalArticle

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    AU - Axford, S.E.

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    AU - Hopwood, D.

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