Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation

Carsten Janke, Jennifer Ortíz, Tomoyuki U. Tanaka, Johannes Lechner, Elmar Schiebel

    Research output: Contribution to journalArticle

    133 Citations (Scopus)

    Abstract

    We show here that Ask1p, Dad2p, Spc19p and Spc34p are subunits of the budding yeast Duo1p-Dam1p- Dad1p complex, which associate with kinetochores and localize along metaphase and anaphase spindles. Analysis of spc34-3 cells revealed three novel functions of the Duo1-Dam1p-Dad1p subunit Spc34p. First, SPC34 is required to establish biorientation of sister kinetochores. Secondly, SPC34 is essential to maintain biorientation. Thirdly, SPC34 is necessary to maintain an anaphase spindle independently of chromosome segregation. Moreover, we show that in spc34-3 cells, sister centromeres preferentially associate with the pre-existing, old spindle pole body (SPB). A similar preferential attachment of sister centromeres to the old SPB occurs in cells depleted of the cohesin Scc1p, a protein with a known role in facilitating biorientation. Thus, the two SPBs are not equally active in early S phase. We suggest that not only in spc34-3 and ?scc1 cells but also in wild-type cells, sister centromeres bind after replication preferentially to microtubules organized by the old SPB. Monopolar attached sister centromeres are resolved to bipolar attachment in wild-type cells but persist in spc34-3 cells.

    Original languageEnglish
    Pages (from-to)181-93
    Number of pages13
    JournalEMBO Journal
    Volume21
    Issue number1-2
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Kinetochores
    Poles
    Centromere
    Spindle Pole Bodies
    Anaphase
    Chromosomes
    Yeast
    Chromosome Segregation
    Saccharomycetales
    Metaphase
    S Phase
    Microtubules
    Proteins

    Cite this

    Janke, Carsten ; Ortíz, Jennifer ; Tanaka, Tomoyuki U. ; Lechner, Johannes ; Schiebel, Elmar. / Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation. In: EMBO Journal. 2002 ; Vol. 21, No. 1-2. pp. 181-93.
    @article{c578ac28b5794fc8829e6c3a81226cf3,
    title = "Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation",
    abstract = "We show here that Ask1p, Dad2p, Spc19p and Spc34p are subunits of the budding yeast Duo1p-Dam1p- Dad1p complex, which associate with kinetochores and localize along metaphase and anaphase spindles. Analysis of spc34-3 cells revealed three novel functions of the Duo1-Dam1p-Dad1p subunit Spc34p. First, SPC34 is required to establish biorientation of sister kinetochores. Secondly, SPC34 is essential to maintain biorientation. Thirdly, SPC34 is necessary to maintain an anaphase spindle independently of chromosome segregation. Moreover, we show that in spc34-3 cells, sister centromeres preferentially associate with the pre-existing, old spindle pole body (SPB). A similar preferential attachment of sister centromeres to the old SPB occurs in cells depleted of the cohesin Scc1p, a protein with a known role in facilitating biorientation. Thus, the two SPBs are not equally active in early S phase. We suggest that not only in spc34-3 and ?scc1 cells but also in wild-type cells, sister centromeres bind after replication preferentially to microtubules organized by the old SPB. Monopolar attached sister centromeres are resolved to bipolar attachment in wild-type cells but persist in spc34-3 cells.",
    author = "Carsten Janke and Jennifer Ort{\'i}z and Tanaka, {Tomoyuki U.} and Johannes Lechner and Elmar Schiebel",
    year = "2002",
    doi = "10.1093/emboj/21.1.181",
    language = "English",
    volume = "21",
    pages = "181--93",
    journal = "EMBO Journal",
    issn = "0261-4189",
    publisher = "EMBO Press",
    number = "1-2",

    }

    Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation. / Janke, Carsten; Ortíz, Jennifer; Tanaka, Tomoyuki U.; Lechner, Johannes; Schiebel, Elmar.

    In: EMBO Journal, Vol. 21, No. 1-2, 2002, p. 181-93.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation

    AU - Janke, Carsten

    AU - Ortíz, Jennifer

    AU - Tanaka, Tomoyuki U.

    AU - Lechner, Johannes

    AU - Schiebel, Elmar

    PY - 2002

    Y1 - 2002

    N2 - We show here that Ask1p, Dad2p, Spc19p and Spc34p are subunits of the budding yeast Duo1p-Dam1p- Dad1p complex, which associate with kinetochores and localize along metaphase and anaphase spindles. Analysis of spc34-3 cells revealed three novel functions of the Duo1-Dam1p-Dad1p subunit Spc34p. First, SPC34 is required to establish biorientation of sister kinetochores. Secondly, SPC34 is essential to maintain biorientation. Thirdly, SPC34 is necessary to maintain an anaphase spindle independently of chromosome segregation. Moreover, we show that in spc34-3 cells, sister centromeres preferentially associate with the pre-existing, old spindle pole body (SPB). A similar preferential attachment of sister centromeres to the old SPB occurs in cells depleted of the cohesin Scc1p, a protein with a known role in facilitating biorientation. Thus, the two SPBs are not equally active in early S phase. We suggest that not only in spc34-3 and ?scc1 cells but also in wild-type cells, sister centromeres bind after replication preferentially to microtubules organized by the old SPB. Monopolar attached sister centromeres are resolved to bipolar attachment in wild-type cells but persist in spc34-3 cells.

    AB - We show here that Ask1p, Dad2p, Spc19p and Spc34p are subunits of the budding yeast Duo1p-Dam1p- Dad1p complex, which associate with kinetochores and localize along metaphase and anaphase spindles. Analysis of spc34-3 cells revealed three novel functions of the Duo1-Dam1p-Dad1p subunit Spc34p. First, SPC34 is required to establish biorientation of sister kinetochores. Secondly, SPC34 is essential to maintain biorientation. Thirdly, SPC34 is necessary to maintain an anaphase spindle independently of chromosome segregation. Moreover, we show that in spc34-3 cells, sister centromeres preferentially associate with the pre-existing, old spindle pole body (SPB). A similar preferential attachment of sister centromeres to the old SPB occurs in cells depleted of the cohesin Scc1p, a protein with a known role in facilitating biorientation. Thus, the two SPBs are not equally active in early S phase. We suggest that not only in spc34-3 and ?scc1 cells but also in wild-type cells, sister centromeres bind after replication preferentially to microtubules organized by the old SPB. Monopolar attached sister centromeres are resolved to bipolar attachment in wild-type cells but persist in spc34-3 cells.

    U2 - 10.1093/emboj/21.1.181

    DO - 10.1093/emboj/21.1.181

    M3 - Article

    C2 - 11782438

    VL - 21

    SP - 181

    EP - 193

    JO - EMBO Journal

    JF - EMBO Journal

    SN - 0261-4189

    IS - 1-2

    ER -