Four zona pellucida glycoproteins are expressed in the human

L. Lefièvre, S. J. Conner, A. Salpekar, O. Olufowobi, P. Ashton, B. Pavlovic, W. Lenton, M. Afnan, I. A. Brewis, M. Monk, D. C. Hughes, C. L. R. Barratt (Lead / Corresponding author)

Research output: Contribution to journalArticle

200 Citations (Scopus)

Abstract

Background: The zona pellucida (ZP) is an extracellular glycoprotein matrix which surrounds all mammalian oocytes. Recent data have shown the presence of four human zona genes (ZP1, ZP2, ZP3 and ZPB). The aim of the study was to determine if all four ZP proteins are expressed and present in the human.

Methods: cDNA derived from human oocytes were used to amplify by PCR the four ZP genes. In addition, isolated native human ZP were heat-solubilized, trypsin-digested and subjected to tandem mass spectrometry (MS/MS).

Results: All four genes were expressed and the respective proteins present in the human ZP. Moreover, a bioinformatics approach showed that the mouse ZPB gene, although present, is likely to encode a non-functional protein.

Conclusions: Four ZP genes are expressed in human oocytes (ZP1, ZP2, ZP3 and ZPB) and preliminary data show that the four corresponding ZP proteins are present in the human ZP. Therefore, this is a fundamental difference with the mouse model

Original languageEnglish
Pages (from-to)1580-1586
Number of pages7
JournalHuman Reproduction
Volume19
Issue number7
DOIs
Publication statusPublished - 1 Jul 2004

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Zona Pellucida
Oocytes
Genes
Proteins
Zona Pellucida Glycoproteins
Herpes Zoster
Tandem Mass Spectrometry
Computational Biology
Population Groups
Trypsin
Extracellular Matrix
Glycoproteins
Complementary DNA
Hot Temperature
Polymerase Chain Reaction

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Computational Biology/methods
  • Egg Proteins/genetics
  • Female
  • Gene Expression
  • Humans
  • Membrane Glycoproteins/genetics
  • Mice/genetics
  • Molecular Sequence Data
  • Oocytes/metabolism
  • Proteomics
  • Receptors, Cell Surface/genetics
  • Zona Pellucida/metabolism
  • Zona Pellucida Glycoproteins

Cite this

Lefièvre, L., Conner, S. J., Salpekar, A., Olufowobi, O., Ashton, P., Pavlovic, B., ... Barratt, C. L. R. (2004). Four zona pellucida glycoproteins are expressed in the human. Human Reproduction, 19(7), 1580-1586. https://doi.org/10.1093/humrep/deh301
Lefièvre, L. ; Conner, S. J. ; Salpekar, A. ; Olufowobi, O. ; Ashton, P. ; Pavlovic, B. ; Lenton, W. ; Afnan, M. ; Brewis, I. A. ; Monk, M. ; Hughes, D. C. ; Barratt, C. L. R. / Four zona pellucida glycoproteins are expressed in the human. In: Human Reproduction. 2004 ; Vol. 19, No. 7. pp. 1580-1586.
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abstract = "Background: The zona pellucida (ZP) is an extracellular glycoprotein matrix which surrounds all mammalian oocytes. Recent data have shown the presence of four human zona genes (ZP1, ZP2, ZP3 and ZPB). The aim of the study was to determine if all four ZP proteins are expressed and present in the human.Methods: cDNA derived from human oocytes were used to amplify by PCR the four ZP genes. In addition, isolated native human ZP were heat-solubilized, trypsin-digested and subjected to tandem mass spectrometry (MS/MS).Results: All four genes were expressed and the respective proteins present in the human ZP. Moreover, a bioinformatics approach showed that the mouse ZPB gene, although present, is likely to encode a non-functional protein.Conclusions: Four ZP genes are expressed in human oocytes (ZP1, ZP2, ZP3 and ZPB) and preliminary data show that the four corresponding ZP proteins are present in the human ZP. Therefore, this is a fundamental difference with the mouse model",
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Lefièvre, L, Conner, SJ, Salpekar, A, Olufowobi, O, Ashton, P, Pavlovic, B, Lenton, W, Afnan, M, Brewis, IA, Monk, M, Hughes, DC & Barratt, CLR 2004, 'Four zona pellucida glycoproteins are expressed in the human', Human Reproduction, vol. 19, no. 7, pp. 1580-1586. https://doi.org/10.1093/humrep/deh301

Four zona pellucida glycoproteins are expressed in the human. / Lefièvre, L.; Conner, S. J.; Salpekar, A.; Olufowobi, O.; Ashton, P.; Pavlovic, B.; Lenton, W.; Afnan, M.; Brewis, I. A.; Monk, M.; Hughes, D. C.; Barratt, C. L. R. (Lead / Corresponding author).

In: Human Reproduction, Vol. 19, No. 7, 01.07.2004, p. 1580-1586.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Four zona pellucida glycoproteins are expressed in the human

AU - Lefièvre, L.

AU - Conner, S. J.

AU - Salpekar, A.

AU - Olufowobi, O.

AU - Ashton, P.

AU - Pavlovic, B.

AU - Lenton, W.

AU - Afnan, M.

AU - Brewis, I. A.

AU - Monk, M.

AU - Hughes, D. C.

AU - Barratt, C. L. R.

PY - 2004/7/1

Y1 - 2004/7/1

N2 - Background: The zona pellucida (ZP) is an extracellular glycoprotein matrix which surrounds all mammalian oocytes. Recent data have shown the presence of four human zona genes (ZP1, ZP2, ZP3 and ZPB). The aim of the study was to determine if all four ZP proteins are expressed and present in the human.Methods: cDNA derived from human oocytes were used to amplify by PCR the four ZP genes. In addition, isolated native human ZP were heat-solubilized, trypsin-digested and subjected to tandem mass spectrometry (MS/MS).Results: All four genes were expressed and the respective proteins present in the human ZP. Moreover, a bioinformatics approach showed that the mouse ZPB gene, although present, is likely to encode a non-functional protein.Conclusions: Four ZP genes are expressed in human oocytes (ZP1, ZP2, ZP3 and ZPB) and preliminary data show that the four corresponding ZP proteins are present in the human ZP. Therefore, this is a fundamental difference with the mouse model

AB - Background: The zona pellucida (ZP) is an extracellular glycoprotein matrix which surrounds all mammalian oocytes. Recent data have shown the presence of four human zona genes (ZP1, ZP2, ZP3 and ZPB). The aim of the study was to determine if all four ZP proteins are expressed and present in the human.Methods: cDNA derived from human oocytes were used to amplify by PCR the four ZP genes. In addition, isolated native human ZP were heat-solubilized, trypsin-digested and subjected to tandem mass spectrometry (MS/MS).Results: All four genes were expressed and the respective proteins present in the human ZP. Moreover, a bioinformatics approach showed that the mouse ZPB gene, although present, is likely to encode a non-functional protein.Conclusions: Four ZP genes are expressed in human oocytes (ZP1, ZP2, ZP3 and ZPB) and preliminary data show that the four corresponding ZP proteins are present in the human ZP. Therefore, this is a fundamental difference with the mouse model

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KW - Animals

KW - Base Sequence

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KW - Egg Proteins/genetics

KW - Female

KW - Gene Expression

KW - Humans

KW - Membrane Glycoproteins/genetics

KW - Mice/genetics

KW - Molecular Sequence Data

KW - Oocytes/metabolism

KW - Proteomics

KW - Receptors, Cell Surface/genetics

KW - Zona Pellucida/metabolism

KW - Zona Pellucida Glycoproteins

U2 - 10.1093/humrep/deh301

DO - 10.1093/humrep/deh301

M3 - Article

C2 - 15142998

VL - 19

SP - 1580

EP - 1586

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - 7

ER -

Lefièvre L, Conner SJ, Salpekar A, Olufowobi O, Ashton P, Pavlovic B et al. Four zona pellucida glycoproteins are expressed in the human. Human Reproduction. 2004 Jul 1;19(7):1580-1586. https://doi.org/10.1093/humrep/deh301