Genetic diversity and genome wide association study of β-glucan content in tetraploid wheat grains

Ilaria Marcotuli, Kelly Houston, Julian G. Schwerdt, Robbie Waugh, Geoffrey B. Fincher, Rachel A. Burton, Antonio Blanco, Agata Gadaleta (Lead / Corresponding author)

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Abstract

Non-starch polysaccharides (NSPs) have many health benefits, including immunomodulatory activity, lowering serum cholesterol, a faecal bulking effect, enhanced absorption of certain minerals, prebiotic effects and the amelioration of type II diabetes. The principal components of the NSP in cereal grains are (1,3;1,4)-β-glucans and arabinoxylans. Although (1,3;1,4)-β-glucan (hereafter called β-glucan) is not the most representative component of wheat cell walls, it is one of the most important types of soluble fibre in terms of its proven beneficial effects on human health. In the present work we explored the genetic variability of β-glucan content in grains from a tetraploid wheat collection that had been genotyped with a 90k-iSelect array, and combined this data to carry out an association analysis. The β-glucan content, expressed as a percentage w/w of grain dry weight, ranged from 0.18% to 0.89% across the collection. Our analysis identified seven genomic regions associated with β-glucan, located on chromosomes 1A, 2A (two), 2B, 5B and 7A (two), confirming the quantitative nature of this trait. Analysis of marker trait associations (MTAs) in syntenic regions of several grass species revealed putative candidate genes that might influence β-glucan levels in the endosperm, possibly via their participation in carbon partitioning. These include the glycosyl hydrolases endo-β-(1,4)-glucanase (cellulase), β-amylase, (1,4)-β-xylan endohydrolase, xylanase inhibitor protein I, isoamylase and the glycosyl transferase starch synthase II.

Original languageEnglish
Article numbere0152590
Number of pages15
JournalPLoS ONE
Volume11
Issue number4
DOIs
Publication statusPublished - 5 Apr 2016

Fingerprint

Tetraploidy
Glucans
Genome-Wide Association Study
glucans
tetraploidy
Triticum
Genes
genetic variation
wheat
Polysaccharides
Isoamylase
Health
Prebiotics
Endosperm
Cellulase
polysaccharides
Hydrolases
Insurance Benefits
Amylases
Chromosomes

Keywords

  • Genetic variation
  • Genome-wide association study
  • Plant proteins
  • Seeds
  • Tetraploidy
  • Triticum
  • beta-glucans
  • Journal article
  • Research support, Non-U.S. Gov't

Cite this

Marcotuli, I., Houston, K., Schwerdt, J. G., Waugh, R., Fincher, G. B., Burton, R. A., ... Gadaleta, A. (2016). Genetic diversity and genome wide association study of β-glucan content in tetraploid wheat grains. PLoS ONE, 11(4), [e0152590]. https://doi.org/10.1371/journal.pone.0152590
Marcotuli, Ilaria ; Houston, Kelly ; Schwerdt, Julian G. ; Waugh, Robbie ; Fincher, Geoffrey B. ; Burton, Rachel A. ; Blanco, Antonio ; Gadaleta, Agata. / Genetic diversity and genome wide association study of β-glucan content in tetraploid wheat grains. In: PLoS ONE. 2016 ; Vol. 11, No. 4.
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Marcotuli, I, Houston, K, Schwerdt, JG, Waugh, R, Fincher, GB, Burton, RA, Blanco, A & Gadaleta, A 2016, 'Genetic diversity and genome wide association study of β-glucan content in tetraploid wheat grains', PLoS ONE, vol. 11, no. 4, e0152590. https://doi.org/10.1371/journal.pone.0152590

Genetic diversity and genome wide association study of β-glucan content in tetraploid wheat grains. / Marcotuli, Ilaria; Houston, Kelly; Schwerdt, Julian G.; Waugh, Robbie; Fincher, Geoffrey B.; Burton, Rachel A.; Blanco, Antonio; Gadaleta, Agata (Lead / Corresponding author).

In: PLoS ONE, Vol. 11, No. 4, e0152590, 05.04.2016.

Research output: Contribution to journalArticle

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T1 - Genetic diversity and genome wide association study of β-glucan content in tetraploid wheat grains

AU - Marcotuli, Ilaria

AU - Houston, Kelly

AU - Schwerdt, Julian G.

AU - Waugh, Robbie

AU - Fincher, Geoffrey B.

AU - Burton, Rachel A.

AU - Blanco, Antonio

AU - Gadaleta, Agata

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AB - Non-starch polysaccharides (NSPs) have many health benefits, including immunomodulatory activity, lowering serum cholesterol, a faecal bulking effect, enhanced absorption of certain minerals, prebiotic effects and the amelioration of type II diabetes. The principal components of the NSP in cereal grains are (1,3;1,4)-β-glucans and arabinoxylans. Although (1,3;1,4)-β-glucan (hereafter called β-glucan) is not the most representative component of wheat cell walls, it is one of the most important types of soluble fibre in terms of its proven beneficial effects on human health. In the present work we explored the genetic variability of β-glucan content in grains from a tetraploid wheat collection that had been genotyped with a 90k-iSelect array, and combined this data to carry out an association analysis. The β-glucan content, expressed as a percentage w/w of grain dry weight, ranged from 0.18% to 0.89% across the collection. Our analysis identified seven genomic regions associated with β-glucan, located on chromosomes 1A, 2A (two), 2B, 5B and 7A (two), confirming the quantitative nature of this trait. Analysis of marker trait associations (MTAs) in syntenic regions of several grass species revealed putative candidate genes that might influence β-glucan levels in the endosperm, possibly via their participation in carbon partitioning. These include the glycosyl hydrolases endo-β-(1,4)-glucanase (cellulase), β-amylase, (1,4)-β-xylan endohydrolase, xylanase inhibitor protein I, isoamylase and the glycosyl transferase starch synthase II.

KW - Genetic variation

KW - Genome-wide association study

KW - Plant proteins

KW - Seeds

KW - Tetraploidy

KW - Triticum

KW - beta-glucans

KW - Journal article

KW - Research support, Non-U.S. Gov't

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DO - 10.1371/journal.pone.0152590

M3 - Article

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JO - PLoS ONE

JF - PLoS ONE

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