Inactivation of glycogen synthase kinase-3β by phosphorylation

New kinase connections in insulin and growth-factor signalling

C. Sutherland, I. A. Leighton, P. Cohen

Research output: Contribution to journalArticle

642 Citations (Scopus)

Abstract

The β-isoform of glycogen synthase kinase-3 (GSK3β) isolated from rabbit skeletal muscle was inactivated 90-95% following incubation with MgATP and either MAP kinase-activated protein kinase-1 (MAPKAP kinase-1, also termed RSK-2) or p70 S6 kinase (p70(S6K)), and re-activated with protein phosphatase 2A. MAPKAP kinase-1 and p70(S6K) phosphorylated the same tryptic peptide on GSK3β and the site of phosphorylation was identified as the serine located nine residues from the N-terminus of the protein. The inhibitory effect of Ser-9 phosphorylation on GSK3β activity was observed with three substrates, (inhibitor-2, c-jun and a synthetic peptide), and also with glycogen synthase provided that 0.15 M KCl was added to the assays. The results suggest that Ser-9 phosphorylation underlies the reported inhibition of GSK3β by insulin and that GSK3 may represent a point of convergence of two major growth-factor-stimulated protein kinase cascades.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalBiochemical Journal
Volume296
Issue number1
DOIs
Publication statusPublished - 15 Nov 1993

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Glycogen Synthase Kinase 3
Phosphorylation
90-kDa Ribosomal Protein S6 Kinases
Intercellular Signaling Peptides and Proteins
Phosphotransferases
Insulin
70-kDa Ribosomal Protein S6 Kinases
Protein Phosphatase 2
Glycogen Synthase
Peptides
Protein Kinases
Serine
Muscle
Assays
Protein Isoforms
Skeletal Muscle
Adenosine Triphosphate
Rabbits
Substrates
Proteins

Cite this

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abstract = "The β-isoform of glycogen synthase kinase-3 (GSK3β) isolated from rabbit skeletal muscle was inactivated 90-95{\%} following incubation with MgATP and either MAP kinase-activated protein kinase-1 (MAPKAP kinase-1, also termed RSK-2) or p70 S6 kinase (p70(S6K)), and re-activated with protein phosphatase 2A. MAPKAP kinase-1 and p70(S6K) phosphorylated the same tryptic peptide on GSK3β and the site of phosphorylation was identified as the serine located nine residues from the N-terminus of the protein. The inhibitory effect of Ser-9 phosphorylation on GSK3β activity was observed with three substrates, (inhibitor-2, c-jun and a synthetic peptide), and also with glycogen synthase provided that 0.15 M KCl was added to the assays. The results suggest that Ser-9 phosphorylation underlies the reported inhibition of GSK3β by insulin and that GSK3 may represent a point of convergence of two major growth-factor-stimulated protein kinase cascades.",
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Inactivation of glycogen synthase kinase-3β by phosphorylation : New kinase connections in insulin and growth-factor signalling. / Sutherland, C.; Leighton, I. A.; Cohen, P.

In: Biochemical Journal, Vol. 296, No. 1, 15.11.1993, p. 15-19.

Research output: Contribution to journalArticle

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AU - Cohen, P.

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