Investigation of platelet glycoprotein IIIa polymorphism using flow cytometry in patients with rheumatoid arthritis

M McLaren, A Waring, B Galarraga, A Rudd, K Morley, J J F Belch

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Previous work has shown that the human platelet antigen (HPA) 1b polymorphism of platelet glycoprotein IIIa (GPIIIa) is implicated in the development of ischaemic vascular disease. HPA1b positive platelets have a lower threshold for activation and may exert a greater thrombotic tendency than those without the 1b allele. However, platelets heterozygous for the polymorphism are also more sensitive to aspirin than those homozygous for the 1b allele, which have a similar sensitivity to those without the 1b allele. A flow cytometric method has become available to identify this polymorphism. The aim of our study was to evaluate the use of this assay in patients with rheumatoid arthritis (RA) and to determine the incidence of the 1b allele in these patients. We also compared platelet aggregation and platelet/white blood cell interaction in patients with or without this polymorphism.
    Original languageEnglish
    Pages (from-to)437-440
    Number of pages4
    JournalScandinavian Journal of Rheumatology
    Volume34
    Issue number6
    DOIs
    Publication statusPublished - 2005

    Fingerprint

    Integrin beta3
    Rheumatoid Arthritis
    Flow Cytometry
    Alleles
    Blood Platelets
    Vascular Diseases
    Platelet Aggregation
    Cell Communication
    Aspirin
    Leukocytes
    Incidence

    Cite this

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    title = "Investigation of platelet glycoprotein IIIa polymorphism using flow cytometry in patients with rheumatoid arthritis",
    abstract = "Previous work has shown that the human platelet antigen (HPA) 1b polymorphism of platelet glycoprotein IIIa (GPIIIa) is implicated in the development of ischaemic vascular disease. HPA1b positive platelets have a lower threshold for activation and may exert a greater thrombotic tendency than those without the 1b allele. However, platelets heterozygous for the polymorphism are also more sensitive to aspirin than those homozygous for the 1b allele, which have a similar sensitivity to those without the 1b allele. A flow cytometric method has become available to identify this polymorphism. The aim of our study was to evaluate the use of this assay in patients with rheumatoid arthritis (RA) and to determine the incidence of the 1b allele in these patients. We also compared platelet aggregation and platelet/white blood cell interaction in patients with or without this polymorphism.",
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    Investigation of platelet glycoprotein IIIa polymorphism using flow cytometry in patients with rheumatoid arthritis. / McLaren, M; Waring, A; Galarraga, B; Rudd, A; Morley, K; Belch, J J F.

    In: Scandinavian Journal of Rheumatology, Vol. 34, No. 6, 2005, p. 437-440.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Investigation of platelet glycoprotein IIIa polymorphism using flow cytometry in patients with rheumatoid arthritis

    AU - McLaren, M

    AU - Waring, A

    AU - Galarraga, B

    AU - Rudd, A

    AU - Morley, K

    AU - Belch, J J F

    PY - 2005

    Y1 - 2005

    N2 - Previous work has shown that the human platelet antigen (HPA) 1b polymorphism of platelet glycoprotein IIIa (GPIIIa) is implicated in the development of ischaemic vascular disease. HPA1b positive platelets have a lower threshold for activation and may exert a greater thrombotic tendency than those without the 1b allele. However, platelets heterozygous for the polymorphism are also more sensitive to aspirin than those homozygous for the 1b allele, which have a similar sensitivity to those without the 1b allele. A flow cytometric method has become available to identify this polymorphism. The aim of our study was to evaluate the use of this assay in patients with rheumatoid arthritis (RA) and to determine the incidence of the 1b allele in these patients. We also compared platelet aggregation and platelet/white blood cell interaction in patients with or without this polymorphism.

    AB - Previous work has shown that the human platelet antigen (HPA) 1b polymorphism of platelet glycoprotein IIIa (GPIIIa) is implicated in the development of ischaemic vascular disease. HPA1b positive platelets have a lower threshold for activation and may exert a greater thrombotic tendency than those without the 1b allele. However, platelets heterozygous for the polymorphism are also more sensitive to aspirin than those homozygous for the 1b allele, which have a similar sensitivity to those without the 1b allele. A flow cytometric method has become available to identify this polymorphism. The aim of our study was to evaluate the use of this assay in patients with rheumatoid arthritis (RA) and to determine the incidence of the 1b allele in these patients. We also compared platelet aggregation and platelet/white blood cell interaction in patients with or without this polymorphism.

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    DO - 10.1080/03009740510026607

    M3 - Article

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    JO - Scandinavian Journal of Rheumatology

    JF - Scandinavian Journal of Rheumatology

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