Localization of cytochrome P-450 gene expression in normal and diseased human liver by in situ hybridization of wax-embedded archival material

Colin N. Palmer, Philip J. Coates, Susan E. Davies, Elizabeth A. Shephard, Ian R. Phillips

    Research output: Contribution to journalArticle

    36 Citations (Scopus)

    Abstract

    The localization of the expression of several cytochrome P-450 genes in normal and diseased human liver was investigated by in situ hybridization of formalin-fixed, paraffin wax-embedded archival tissue samples with 35S-labeled antisense RNA probes. The results demonstrated that genes coding for members of the cytochrome P-450 3A subfamily (CYP3A) were preferentially expressed in hepatocytes in acinar zone 3 (the centrilobular region), whereas genes coding for CYP1A2, CYP2A, 2B and 2C were expressed uniformly throughout the liver acinus. In cirrhotic livers, CYP2A and 2B genes (and to a lesser extent, CYP3A genes) were highly expressed in isolated hepatocytes located at the junction of parenchyma with fibrous septa. The cause and significance of the position-dependent expression of specific cytochrome P-450 genes in normal and diseased human liver are discussed.
    Original languageEnglish
    Pages (from-to)682-687
    Number of pages6
    JournalHepatology
    Volume16
    Issue number3
    DOIs
    Publication statusPublished - 1992

    Fingerprint

    Waxes
    Cytochrome P-450 Enzyme System
    In Situ Hybridization
    Liver Diseases
    Gene Expression
    Genes
    Hepatocytes
    RNA Probes
    Antisense RNA
    Cytochrome P-450 CYP1A2
    Liver
    Paraffin
    Formaldehyde

    Cite this

    Palmer, Colin N. ; Coates, Philip J. ; Davies, Susan E. ; Shephard, Elizabeth A. ; Phillips, Ian R. / Localization of cytochrome P-450 gene expression in normal and diseased human liver by in situ hybridization of wax-embedded archival material. In: Hepatology. 1992 ; Vol. 16, No. 3. pp. 682-687.
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    abstract = "The localization of the expression of several cytochrome P-450 genes in normal and diseased human liver was investigated by in situ hybridization of formalin-fixed, paraffin wax-embedded archival tissue samples with 35S-labeled antisense RNA probes. The results demonstrated that genes coding for members of the cytochrome P-450 3A subfamily (CYP3A) were preferentially expressed in hepatocytes in acinar zone 3 (the centrilobular region), whereas genes coding for CYP1A2, CYP2A, 2B and 2C were expressed uniformly throughout the liver acinus. In cirrhotic livers, CYP2A and 2B genes (and to a lesser extent, CYP3A genes) were highly expressed in isolated hepatocytes located at the junction of parenchyma with fibrous septa. The cause and significance of the position-dependent expression of specific cytochrome P-450 genes in normal and diseased human liver are discussed.",
    author = "Palmer, {Colin N.} and Coates, {Philip J.} and Davies, {Susan E.} and Shephard, {Elizabeth A.} and Phillips, {Ian R.}",
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    Localization of cytochrome P-450 gene expression in normal and diseased human liver by in situ hybridization of wax-embedded archival material. / Palmer, Colin N.; Coates, Philip J.; Davies, Susan E.; Shephard, Elizabeth A.; Phillips, Ian R.

    In: Hepatology, Vol. 16, No. 3, 1992, p. 682-687.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Localization of cytochrome P-450 gene expression in normal and diseased human liver by in situ hybridization of wax-embedded archival material

    AU - Palmer, Colin N.

    AU - Coates, Philip J.

    AU - Davies, Susan E.

    AU - Shephard, Elizabeth A.

    AU - Phillips, Ian R.

    PY - 1992

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    AB - The localization of the expression of several cytochrome P-450 genes in normal and diseased human liver was investigated by in situ hybridization of formalin-fixed, paraffin wax-embedded archival tissue samples with 35S-labeled antisense RNA probes. The results demonstrated that genes coding for members of the cytochrome P-450 3A subfamily (CYP3A) were preferentially expressed in hepatocytes in acinar zone 3 (the centrilobular region), whereas genes coding for CYP1A2, CYP2A, 2B and 2C were expressed uniformly throughout the liver acinus. In cirrhotic livers, CYP2A and 2B genes (and to a lesser extent, CYP3A genes) were highly expressed in isolated hepatocytes located at the junction of parenchyma with fibrous septa. The cause and significance of the position-dependent expression of specific cytochrome P-450 genes in normal and diseased human liver are discussed.

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    DO - 10.1002/hep.1840160311

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