Mannosamine can replace glucosamine in glycosylphosphatidylinositols of Plasmodium falciparum in vitro

Nahid Azzouz, Cristiana Santos de Macedo, Michael A. J. Ferguson, Terry K. Smith, Ralph T. Schwarz

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Mannosamine (2-deoxy-2-amino-D-mannose) is unable to block GP1 biosynthesis in neither parasite development nor GPI biosynthesis were blocked by mannosamine treatment in P. falciparum cultures. Further, it was shown by metabolic labeling with [H-3]mannosamine and subsequent monosaccharide analysis by high pH anion exchange chromatography that mannosamine is converted at a high rate into glucosamine. Both mannosamine and glucosamine are incorporated into P.falciparum glycolipids, but the characterization of mannosamine-labeled glycolipids synthesized in vivo proved difficult. Therefore. it cell-free system was developed to investigate the incorporation of [H-3]mannosamine into glycolipids in P falciparum. It was observed that mannosamine is incorporated in vitro into P falciparum glycolipids, which possess a phosphate group. Chemical (nitrous acid deamination, mild acid hydrolysis and alkaline hydrolysis) and enzymatic (PI-PLC) treatments of [H-3]mannosamine-labeled glycolipids synthesized in vitro showed the presence or GPIs. Further analyses by Bio-Gel P4 size-exclusion chromatography and HPAEC demonstrated the presence of a mannosamine-containing GPI-like structures, where mannosamine is incorporated instead of glucosamine, i.e. Man(3)-ManN-PI, This utilization of rnannosamine is novel and not been described for any other cellular or parasitic system. (c) 2005 Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)12-24
    Number of pages13
    JournalMolecular and Biochemical Parasitology
    Volume142
    Issue number1
    DOIs
    Publication statusPublished - Jul 2005

    Cite this

    Azzouz, Nahid ; de Macedo, Cristiana Santos ; Ferguson, Michael A. J. ; Smith, Terry K. ; Schwarz, Ralph T. . / Mannosamine can replace glucosamine in glycosylphosphatidylinositols of Plasmodium falciparum in vitro. In: Molecular and Biochemical Parasitology. 2005 ; Vol. 142, No. 1. pp. 12-24.
    @article{5b9bd4d2650248a9af0b245095d005cf,
    title = "Mannosamine can replace glucosamine in glycosylphosphatidylinositols of Plasmodium falciparum in vitro",
    abstract = "Mannosamine (2-deoxy-2-amino-D-mannose) is unable to block GP1 biosynthesis in neither parasite development nor GPI biosynthesis were blocked by mannosamine treatment in P. falciparum cultures. Further, it was shown by metabolic labeling with [H-3]mannosamine and subsequent monosaccharide analysis by high pH anion exchange chromatography that mannosamine is converted at a high rate into glucosamine. Both mannosamine and glucosamine are incorporated into P.falciparum glycolipids, but the characterization of mannosamine-labeled glycolipids synthesized in vivo proved difficult. Therefore. it cell-free system was developed to investigate the incorporation of [H-3]mannosamine into glycolipids in P falciparum. It was observed that mannosamine is incorporated in vitro into P falciparum glycolipids, which possess a phosphate group. Chemical (nitrous acid deamination, mild acid hydrolysis and alkaline hydrolysis) and enzymatic (PI-PLC) treatments of [H-3]mannosamine-labeled glycolipids synthesized in vitro showed the presence or GPIs. Further analyses by Bio-Gel P4 size-exclusion chromatography and HPAEC demonstrated the presence of a mannosamine-containing GPI-like structures, where mannosamine is incorporated instead of glucosamine, i.e. Man(3)-ManN-PI, This utilization of rnannosamine is novel and not been described for any other cellular or parasitic system. (c) 2005 Elsevier B.V. All rights reserved.",
    author = "Nahid Azzouz and {de Macedo}, {Cristiana Santos} and Ferguson, {Michael A. J.} and Smith, {Terry K.} and Schwarz, {Ralph T.}",
    year = "2005",
    month = "7",
    doi = "10.1016/j.molbiopara.2005.02.011",
    language = "English",
    volume = "142",
    pages = "12--24",
    journal = "Molecular and Biochemical Parasitology",
    issn = "0166-6851",
    publisher = "Elsevier",
    number = "1",

    }

    Mannosamine can replace glucosamine in glycosylphosphatidylinositols of Plasmodium falciparum in vitro. / Azzouz, Nahid; de Macedo, Cristiana Santos; Ferguson, Michael A. J. ; Smith, Terry K. ; Schwarz, Ralph T. .

    In: Molecular and Biochemical Parasitology, Vol. 142, No. 1, 07.2005, p. 12-24.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Mannosamine can replace glucosamine in glycosylphosphatidylinositols of Plasmodium falciparum in vitro

    AU - Azzouz, Nahid

    AU - de Macedo, Cristiana Santos

    AU - Ferguson, Michael A. J.

    AU - Smith, Terry K.

    AU - Schwarz, Ralph T.

    PY - 2005/7

    Y1 - 2005/7

    N2 - Mannosamine (2-deoxy-2-amino-D-mannose) is unable to block GP1 biosynthesis in neither parasite development nor GPI biosynthesis were blocked by mannosamine treatment in P. falciparum cultures. Further, it was shown by metabolic labeling with [H-3]mannosamine and subsequent monosaccharide analysis by high pH anion exchange chromatography that mannosamine is converted at a high rate into glucosamine. Both mannosamine and glucosamine are incorporated into P.falciparum glycolipids, but the characterization of mannosamine-labeled glycolipids synthesized in vivo proved difficult. Therefore. it cell-free system was developed to investigate the incorporation of [H-3]mannosamine into glycolipids in P falciparum. It was observed that mannosamine is incorporated in vitro into P falciparum glycolipids, which possess a phosphate group. Chemical (nitrous acid deamination, mild acid hydrolysis and alkaline hydrolysis) and enzymatic (PI-PLC) treatments of [H-3]mannosamine-labeled glycolipids synthesized in vitro showed the presence or GPIs. Further analyses by Bio-Gel P4 size-exclusion chromatography and HPAEC demonstrated the presence of a mannosamine-containing GPI-like structures, where mannosamine is incorporated instead of glucosamine, i.e. Man(3)-ManN-PI, This utilization of rnannosamine is novel and not been described for any other cellular or parasitic system. (c) 2005 Elsevier B.V. All rights reserved.

    AB - Mannosamine (2-deoxy-2-amino-D-mannose) is unable to block GP1 biosynthesis in neither parasite development nor GPI biosynthesis were blocked by mannosamine treatment in P. falciparum cultures. Further, it was shown by metabolic labeling with [H-3]mannosamine and subsequent monosaccharide analysis by high pH anion exchange chromatography that mannosamine is converted at a high rate into glucosamine. Both mannosamine and glucosamine are incorporated into P.falciparum glycolipids, but the characterization of mannosamine-labeled glycolipids synthesized in vivo proved difficult. Therefore. it cell-free system was developed to investigate the incorporation of [H-3]mannosamine into glycolipids in P falciparum. It was observed that mannosamine is incorporated in vitro into P falciparum glycolipids, which possess a phosphate group. Chemical (nitrous acid deamination, mild acid hydrolysis and alkaline hydrolysis) and enzymatic (PI-PLC) treatments of [H-3]mannosamine-labeled glycolipids synthesized in vitro showed the presence or GPIs. Further analyses by Bio-Gel P4 size-exclusion chromatography and HPAEC demonstrated the presence of a mannosamine-containing GPI-like structures, where mannosamine is incorporated instead of glucosamine, i.e. Man(3)-ManN-PI, This utilization of rnannosamine is novel and not been described for any other cellular or parasitic system. (c) 2005 Elsevier B.V. All rights reserved.

    U2 - 10.1016/j.molbiopara.2005.02.011

    DO - 10.1016/j.molbiopara.2005.02.011

    M3 - Article

    VL - 142

    SP - 12

    EP - 24

    JO - Molecular and Biochemical Parasitology

    JF - Molecular and Biochemical Parasitology

    SN - 0166-6851

    IS - 1

    ER -