Mitogen and stress-activated kinases 1/2 regulate ischemia-induced hippocampal progenitor cell proliferation and neurogenesis

K. Karelina, Y. Liu, D. Alzate-Correa, K. L. Wheaton, K. R. Hoyt, J. S. C. Arthur, K. Obrietan (Lead / Corresponding author)

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    Abstract

    Pathophysiological conditions such as cerebral ischemia trigger the production of new neurons from the neurogenic niche within the subgranular zone (SGZ) of the dentate gyrus. The functional significance of ischemia-induced neurogenesis is believed to be the regeneration of lost cells, thus contributing to post-ischemia recovery. However, the cell signaling mechanisms by which this process is regulated are still under investigation. Here, we investigated the role of mitogen and stress-activated protein kinases (MSK1/2) in the regulation of progenitor cell proliferation and neurogenesis after cerebral ischemia. Using the endothelin-1 model of ischemia, wild-type (WT) and MSK1(-/-)/MSK2(-/-) (MSK dKO) mice were injected with BrdU and sacrificed 2days, 4weeks, or 6weeks later for the analysis of progenitor cell proliferation, neurogenesis, and neuronal morphology, respectively. We report a decrease in SGZ progenitor cell proliferation in MSK dKO mice compared to WT mice. Moreover, MSK dKO mice exhibited reduced neurogenesis and a delayed maturation of ischemia-induced newborn neurons. Further, structural analysis of neuronal arborization revealed reduced branching complexity in MSK dKO compared to WT mice. Taken together, this dataset suggests that MSK1/2 plays a significant role in the regulation of ischemia-induced progenitor cell proliferation and neurogenesis. Ultimately, revealing the cell signaling mechanisms that promote neuronal recovery will lead to novel pharmacological approaches for the treatment of neurodegenerative diseases such as cerebral ischemia.

    Original languageEnglish
    Pages (from-to)292-302
    Number of pages11
    JournalNeuroscience
    Volume285
    DOIs
    Publication statusPublished - 29 Jan 2015

    Fingerprint

    Neurogenesis
    Mitogens
    Phosphotransferases
    Stem Cells
    Ischemia
    Cell Proliferation
    Brain Ischemia
    Mitogen-Activated Protein Kinase 11
    Neurons
    Neuronal Plasticity
    Dentate Gyrus
    Endothelin-1
    Bromodeoxyuridine
    Mitogen-Activated Protein Kinases
    Neurodegenerative Diseases
    Regeneration
    Pharmacology

    Cite this

    Karelina, K. ; Liu, Y. ; Alzate-Correa, D. ; Wheaton, K. L. ; Hoyt, K. R. ; Arthur, J. S. C. ; Obrietan, K. / Mitogen and stress-activated kinases 1/2 regulate ischemia-induced hippocampal progenitor cell proliferation and neurogenesis. In: Neuroscience. 2015 ; Vol. 285. pp. 292-302.
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    abstract = "Pathophysiological conditions such as cerebral ischemia trigger the production of new neurons from the neurogenic niche within the subgranular zone (SGZ) of the dentate gyrus. The functional significance of ischemia-induced neurogenesis is believed to be the regeneration of lost cells, thus contributing to post-ischemia recovery. However, the cell signaling mechanisms by which this process is regulated are still under investigation. Here, we investigated the role of mitogen and stress-activated protein kinases (MSK1/2) in the regulation of progenitor cell proliferation and neurogenesis after cerebral ischemia. Using the endothelin-1 model of ischemia, wild-type (WT) and MSK1(-/-)/MSK2(-/-) (MSK dKO) mice were injected with BrdU and sacrificed 2days, 4weeks, or 6weeks later for the analysis of progenitor cell proliferation, neurogenesis, and neuronal morphology, respectively. We report a decrease in SGZ progenitor cell proliferation in MSK dKO mice compared to WT mice. Moreover, MSK dKO mice exhibited reduced neurogenesis and a delayed maturation of ischemia-induced newborn neurons. Further, structural analysis of neuronal arborization revealed reduced branching complexity in MSK dKO compared to WT mice. Taken together, this dataset suggests that MSK1/2 plays a significant role in the regulation of ischemia-induced progenitor cell proliferation and neurogenesis. Ultimately, revealing the cell signaling mechanisms that promote neuronal recovery will lead to novel pharmacological approaches for the treatment of neurodegenerative diseases such as cerebral ischemia.",
    author = "K. Karelina and Y. Liu and D. Alzate-Correa and Wheaton, {K. L.} and Hoyt, {K. R.} and Arthur, {J. S. C.} and K. Obrietan",
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    Mitogen and stress-activated kinases 1/2 regulate ischemia-induced hippocampal progenitor cell proliferation and neurogenesis. / Karelina, K.; Liu, Y.; Alzate-Correa, D.; Wheaton, K. L.; Hoyt, K. R.; Arthur, J. S. C.; Obrietan, K. (Lead / Corresponding author).

    In: Neuroscience, Vol. 285, 29.01.2015, p. 292-302.

    Research output: Contribution to journalArticle

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    AU - Karelina, K.

    AU - Liu, Y.

    AU - Alzate-Correa, D.

    AU - Wheaton, K. L.

    AU - Hoyt, K. R.

    AU - Arthur, J. S. C.

    AU - Obrietan, K.

    N1 - Copyright © 2014 IBRO. Published by Elsevier Ltd. All rights reserved.

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