Molecular characterization of two ammonium transporters from the ectomycorrhizal fungus Hebeloma cylindrosporum

Arnaud Javelle, Blanca-Rosa Rodríguez-Pastrana, Christophe Jacob, Bernard Botton, Annick Brun, Bruno André, Anne-Marie Marini, Michel Chalot

    Research output: Contribution to journalArticle

    46 Citations (Scopus)

    Abstract

    Heterologous expression of the yeast triple Mep mutant has enabled the first molecular characterization of AMT/MEP family members in an ectomycorrhizal fungus. External hyphae, which play a key role in nitrogen nutrition of trees, are considered as the absorbing structure of the ectomycorrhizal symbiosis and therefore molecular studies on ammonium transport in hyphae are urgently needed. The kinetic properties of AMT2 and AMT3 from Hebeloma cylindrosporum were studied in Saccharomyces cerevisiae. Expression of HcAmts in the yeast triple Mep mutant restored ammonium retention within cells. The HcAmts did not complement the ammonium sensing defect phenotype of Mep2Delta cells during pseudohyphal differentiation. Northern blot analysis in H. cylindrosporum showed that the HcAMTs were up-regulated upon nitrogen deprivation and down-regulated by ammonium.
    Original languageEnglish
    Pages (from-to)393-8
    Number of pages6
    JournalFEBS Letters
    Volume505
    Issue number3
    DOIs
    Publication statusPublished - 2001

    Fingerprint

    Hebeloma
    Fungi
    Ammonium Compounds
    Yeast
    Hyphae
    Nitrogen
    Yeasts
    Symbiosis
    Nutrition
    Northern Blotting
    Saccharomyces cerevisiae
    Phenotype
    Defects
    Kinetics

    Cite this

    Javelle, A., Rodríguez-Pastrana, B-R., Jacob, C., Botton, B., Brun, A., André, B., ... Chalot, M. (2001). Molecular characterization of two ammonium transporters from the ectomycorrhizal fungus Hebeloma cylindrosporum. FEBS Letters, 505(3), 393-8. https://doi.org/10.1016/S0014-5793(01)02802-2
    Javelle, Arnaud ; Rodríguez-Pastrana, Blanca-Rosa ; Jacob, Christophe ; Botton, Bernard ; Brun, Annick ; André, Bruno ; Marini, Anne-Marie ; Chalot, Michel. / Molecular characterization of two ammonium transporters from the ectomycorrhizal fungus Hebeloma cylindrosporum. In: FEBS Letters. 2001 ; Vol. 505, No. 3. pp. 393-8.
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    abstract = "Heterologous expression of the yeast triple Mep mutant has enabled the first molecular characterization of AMT/MEP family members in an ectomycorrhizal fungus. External hyphae, which play a key role in nitrogen nutrition of trees, are considered as the absorbing structure of the ectomycorrhizal symbiosis and therefore molecular studies on ammonium transport in hyphae are urgently needed. The kinetic properties of AMT2 and AMT3 from Hebeloma cylindrosporum were studied in Saccharomyces cerevisiae. Expression of HcAmts in the yeast triple Mep mutant restored ammonium retention within cells. The HcAmts did not complement the ammonium sensing defect phenotype of Mep2Delta cells during pseudohyphal differentiation. Northern blot analysis in H. cylindrosporum showed that the HcAMTs were up-regulated upon nitrogen deprivation and down-regulated by ammonium.",
    author = "Arnaud Javelle and Blanca-Rosa Rodr{\'i}guez-Pastrana and Christophe Jacob and Bernard Botton and Annick Brun and Bruno Andr{\'e} and Anne-Marie Marini and Michel Chalot",
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    Javelle, A, Rodríguez-Pastrana, B-R, Jacob, C, Botton, B, Brun, A, André, B, Marini, A-M & Chalot, M 2001, 'Molecular characterization of two ammonium transporters from the ectomycorrhizal fungus Hebeloma cylindrosporum', FEBS Letters, vol. 505, no. 3, pp. 393-8. https://doi.org/10.1016/S0014-5793(01)02802-2

    Molecular characterization of two ammonium transporters from the ectomycorrhizal fungus Hebeloma cylindrosporum. / Javelle, Arnaud; Rodríguez-Pastrana, Blanca-Rosa; Jacob, Christophe; Botton, Bernard; Brun, Annick; André, Bruno; Marini, Anne-Marie; Chalot, Michel.

    In: FEBS Letters, Vol. 505, No. 3, 2001, p. 393-8.

    Research output: Contribution to journalArticle

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    AU - Javelle, Arnaud

    AU - Rodríguez-Pastrana, Blanca-Rosa

    AU - Jacob, Christophe

    AU - Botton, Bernard

    AU - Brun, Annick

    AU - André, Bruno

    AU - Marini, Anne-Marie

    AU - Chalot, Michel

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