Molecular profiling of multiplexed gene markers to assess viability of ex vivo human colon explant cultures

Janice E. Drew (Lead / Corresponding author), Andrew J. Farquharson, Hollie Vase, Frank A. Carey, Robert J. C. Steele, Ruth A. Ross, David C. Bunton

    Research output: Contribution to journalArticle

    20 Downloads (Pure)

    Abstract

    Human colon tissue explant culture provides a physiologically relevant model system to study human gut biology. However, the small (20-30 mg) and complex tissue samples used present challenges for monitoring tissue stability, viability, and provision of sufficient tissue for analyses. Combining molecular profiling with explant culture has potential to overcome such limitations, permitting interrogation of complex gene regulation required to maintain gut mucosa in culture, monitor responses to culture environments and interventions. Human ex vivo colon explant gene expression profiles were assayed using an in-house custom-designed hCellMarkerPlex assay at culture time points 0, 1, 2, 4, and 14 h. Characteristic profiles of epithelial cell markers linked to differentiation, cellular polarization, and apoptosis were correlated with visible histochemical changes in explant epithelium during culture and tissue donors. The GenomeLab System provides effective assay of multiple targets not possible from small tissue samples with conventional gene expression technology platforms. This is advantageous to increase the utility of the ex vivo human colon model in applications to interrogate this complex and dynamic tissue environment for use in analytical testing.

    Original languageEnglish
    Pages (from-to)425-430
    Number of pages6
    JournalBioResearch Open Access
    Volume4
    Issue number1
    DOIs
    Publication statusPublished - Nov 2015

    Fingerprint

    Cell culture
    Colon
    Genes
    Tissue
    Gene expression
    Assays
    Tissue Survival
    Tissue culture
    Transcriptome
    Mucous Membrane
    Epithelium
    Epithelial Cells
    Tissue Donors
    Apoptosis
    Technology
    Gene Expression
    Polarization
    Monitoring
    Testing

    Cite this

    Drew, Janice E. ; Farquharson, Andrew J. ; Vase, Hollie ; Carey, Frank A. ; Steele, Robert J. C. ; Ross, Ruth A. ; Bunton, David C. / Molecular profiling of multiplexed gene markers to assess viability of ex vivo human colon explant cultures. In: BioResearch Open Access. 2015 ; Vol. 4, No. 1. pp. 425-430.
    @article{a20722725ea44051a676640411e95a32,
    title = "Molecular profiling of multiplexed gene markers to assess viability of ex vivo human colon explant cultures",
    abstract = "Human colon tissue explant culture provides a physiologically relevant model system to study human gut biology. However, the small (20-30 mg) and complex tissue samples used present challenges for monitoring tissue stability, viability, and provision of sufficient tissue for analyses. Combining molecular profiling with explant culture has potential to overcome such limitations, permitting interrogation of complex gene regulation required to maintain gut mucosa in culture, monitor responses to culture environments and interventions. Human ex vivo colon explant gene expression profiles were assayed using an in-house custom-designed hCellMarkerPlex assay at culture time points 0, 1, 2, 4, and 14 h. Characteristic profiles of epithelial cell markers linked to differentiation, cellular polarization, and apoptosis were correlated with visible histochemical changes in explant epithelium during culture and tissue donors. The GenomeLab System provides effective assay of multiple targets not possible from small tissue samples with conventional gene expression technology platforms. This is advantageous to increase the utility of the ex vivo human colon model in applications to interrogate this complex and dynamic tissue environment for use in analytical testing.",
    author = "Drew, {Janice E.} and Farquharson, {Andrew J.} and Hollie Vase and Carey, {Frank A.} and Steele, {Robert J. C.} and Ross, {Ruth A.} and Bunton, {David C.}",
    year = "2015",
    month = "11",
    doi = "10.1089/biores.2015.0029",
    language = "English",
    volume = "4",
    pages = "425--430",
    journal = "BioResearch Open Access",
    issn = "2164-7844",
    publisher = "Mary Ann Liebert, Inc., publishers",
    number = "1",

    }

    Molecular profiling of multiplexed gene markers to assess viability of ex vivo human colon explant cultures. / Drew, Janice E. (Lead / Corresponding author); Farquharson, Andrew J.; Vase, Hollie; Carey, Frank A.; Steele, Robert J. C.; Ross, Ruth A.; Bunton, David C.

    In: BioResearch Open Access, Vol. 4, No. 1, 11.2015, p. 425-430.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Molecular profiling of multiplexed gene markers to assess viability of ex vivo human colon explant cultures

    AU - Drew, Janice E.

    AU - Farquharson, Andrew J.

    AU - Vase, Hollie

    AU - Carey, Frank A.

    AU - Steele, Robert J. C.

    AU - Ross, Ruth A.

    AU - Bunton, David C.

    PY - 2015/11

    Y1 - 2015/11

    N2 - Human colon tissue explant culture provides a physiologically relevant model system to study human gut biology. However, the small (20-30 mg) and complex tissue samples used present challenges for monitoring tissue stability, viability, and provision of sufficient tissue for analyses. Combining molecular profiling with explant culture has potential to overcome such limitations, permitting interrogation of complex gene regulation required to maintain gut mucosa in culture, monitor responses to culture environments and interventions. Human ex vivo colon explant gene expression profiles were assayed using an in-house custom-designed hCellMarkerPlex assay at culture time points 0, 1, 2, 4, and 14 h. Characteristic profiles of epithelial cell markers linked to differentiation, cellular polarization, and apoptosis were correlated with visible histochemical changes in explant epithelium during culture and tissue donors. The GenomeLab System provides effective assay of multiple targets not possible from small tissue samples with conventional gene expression technology platforms. This is advantageous to increase the utility of the ex vivo human colon model in applications to interrogate this complex and dynamic tissue environment for use in analytical testing.

    AB - Human colon tissue explant culture provides a physiologically relevant model system to study human gut biology. However, the small (20-30 mg) and complex tissue samples used present challenges for monitoring tissue stability, viability, and provision of sufficient tissue for analyses. Combining molecular profiling with explant culture has potential to overcome such limitations, permitting interrogation of complex gene regulation required to maintain gut mucosa in culture, monitor responses to culture environments and interventions. Human ex vivo colon explant gene expression profiles were assayed using an in-house custom-designed hCellMarkerPlex assay at culture time points 0, 1, 2, 4, and 14 h. Characteristic profiles of epithelial cell markers linked to differentiation, cellular polarization, and apoptosis were correlated with visible histochemical changes in explant epithelium during culture and tissue donors. The GenomeLab System provides effective assay of multiple targets not possible from small tissue samples with conventional gene expression technology platforms. This is advantageous to increase the utility of the ex vivo human colon model in applications to interrogate this complex and dynamic tissue environment for use in analytical testing.

    U2 - 10.1089/biores.2015.0029

    DO - 10.1089/biores.2015.0029

    M3 - Article

    VL - 4

    SP - 425

    EP - 430

    JO - BioResearch Open Access

    JF - BioResearch Open Access

    SN - 2164-7844

    IS - 1

    ER -