Persistence of Epstein-Barr virus in Reed-Sternberg cells throughout the course of Hodgkin's disease

Philip J. Coates, Gerard Slavin, A. Jane D'Ardenne

    Research output: Contribution to journalArticle

    48 Citations (Scopus)

    Abstract

    Non-isotopic in situ hybridization employing digoxigenin-labelled DNA probes has been used to localize Epstein-Barr virus (EBV) in 55 cases of Hodgkin's disease (HD). The virus was found in Reed-Sternberg (RS) and mononuclear Hodgkin's cells in nine patients (16 per cent). Further samples taken at different times from three patients also showed the presence of EBV in the malignant cell population. Estimations of the number of EBV genomes present per cell suggested wide variations between different patients, but relatively constant amounts in different samples from the same patient. These findings are compatible with a stable infection of the neoplastic cells and support the notion that EBV may play a role in the development of HD in these patients. We also found evidence for the presence of EBV in a small percentage of non-neoplastic cells in 8 of the 55 samples. This suggests that isolation of EBV from HD tissue does not always signify a pathogenetic role for the virus. Furthermore, it is apparent that a high percentage of HD tissues do not contain demonstrable EBV, and the virus is therefore unlikely to be a causative agent for all cases of HD.
    Original languageEnglish
    Pages (from-to)291-297
    Number of pages7
    JournalJournal of Pathology
    Volume164
    Issue number4
    DOIs
    Publication statusPublished - 1991

    Fingerprint

    Reed-Sternberg Cells
    Human Herpesvirus 4
    Hodgkin Disease
    Viruses
    Digoxigenin
    DNA Probes
    Virus Diseases
    In Situ Hybridization
    Genome
    Infection

    Cite this

    Coates, Philip J. ; Slavin, Gerard ; D'Ardenne, A. Jane. / Persistence of Epstein-Barr virus in Reed-Sternberg cells throughout the course of Hodgkin's disease. In: Journal of Pathology. 1991 ; Vol. 164, No. 4. pp. 291-297.
    @article{4772d4d89f714a20a8e958145b36ddd1,
    title = "Persistence of Epstein-Barr virus in Reed-Sternberg cells throughout the course of Hodgkin's disease",
    abstract = "Non-isotopic in situ hybridization employing digoxigenin-labelled DNA probes has been used to localize Epstein-Barr virus (EBV) in 55 cases of Hodgkin's disease (HD). The virus was found in Reed-Sternberg (RS) and mononuclear Hodgkin's cells in nine patients (16 per cent). Further samples taken at different times from three patients also showed the presence of EBV in the malignant cell population. Estimations of the number of EBV genomes present per cell suggested wide variations between different patients, but relatively constant amounts in different samples from the same patient. These findings are compatible with a stable infection of the neoplastic cells and support the notion that EBV may play a role in the development of HD in these patients. We also found evidence for the presence of EBV in a small percentage of non-neoplastic cells in 8 of the 55 samples. This suggests that isolation of EBV from HD tissue does not always signify a pathogenetic role for the virus. Furthermore, it is apparent that a high percentage of HD tissues do not contain demonstrable EBV, and the virus is therefore unlikely to be a causative agent for all cases of HD.",
    author = "Coates, {Philip J.} and Gerard Slavin and D'Ardenne, {A. Jane}",
    year = "1991",
    doi = "10.1002/path.1711640404",
    language = "English",
    volume = "164",
    pages = "291--297",
    journal = "Journal of Pathology",
    issn = "0022-3417",
    publisher = "Wiley",
    number = "4",

    }

    Persistence of Epstein-Barr virus in Reed-Sternberg cells throughout the course of Hodgkin's disease. / Coates, Philip J.; Slavin, Gerard; D'Ardenne, A. Jane.

    In: Journal of Pathology, Vol. 164, No. 4, 1991, p. 291-297.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Persistence of Epstein-Barr virus in Reed-Sternberg cells throughout the course of Hodgkin's disease

    AU - Coates, Philip J.

    AU - Slavin, Gerard

    AU - D'Ardenne, A. Jane

    PY - 1991

    Y1 - 1991

    N2 - Non-isotopic in situ hybridization employing digoxigenin-labelled DNA probes has been used to localize Epstein-Barr virus (EBV) in 55 cases of Hodgkin's disease (HD). The virus was found in Reed-Sternberg (RS) and mononuclear Hodgkin's cells in nine patients (16 per cent). Further samples taken at different times from three patients also showed the presence of EBV in the malignant cell population. Estimations of the number of EBV genomes present per cell suggested wide variations between different patients, but relatively constant amounts in different samples from the same patient. These findings are compatible with a stable infection of the neoplastic cells and support the notion that EBV may play a role in the development of HD in these patients. We also found evidence for the presence of EBV in a small percentage of non-neoplastic cells in 8 of the 55 samples. This suggests that isolation of EBV from HD tissue does not always signify a pathogenetic role for the virus. Furthermore, it is apparent that a high percentage of HD tissues do not contain demonstrable EBV, and the virus is therefore unlikely to be a causative agent for all cases of HD.

    AB - Non-isotopic in situ hybridization employing digoxigenin-labelled DNA probes has been used to localize Epstein-Barr virus (EBV) in 55 cases of Hodgkin's disease (HD). The virus was found in Reed-Sternberg (RS) and mononuclear Hodgkin's cells in nine patients (16 per cent). Further samples taken at different times from three patients also showed the presence of EBV in the malignant cell population. Estimations of the number of EBV genomes present per cell suggested wide variations between different patients, but relatively constant amounts in different samples from the same patient. These findings are compatible with a stable infection of the neoplastic cells and support the notion that EBV may play a role in the development of HD in these patients. We also found evidence for the presence of EBV in a small percentage of non-neoplastic cells in 8 of the 55 samples. This suggests that isolation of EBV from HD tissue does not always signify a pathogenetic role for the virus. Furthermore, it is apparent that a high percentage of HD tissues do not contain demonstrable EBV, and the virus is therefore unlikely to be a causative agent for all cases of HD.

    U2 - 10.1002/path.1711640404

    DO - 10.1002/path.1711640404

    M3 - Article

    VL - 164

    SP - 291

    EP - 297

    JO - Journal of Pathology

    JF - Journal of Pathology

    SN - 0022-3417

    IS - 4

    ER -