Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus

Ramzi Azzam, Susan L. Chen, Wenying Shou, Angie S. Mah, Gabriela Alexandru, Kim Nasmyth, Roland S. Annan, Steven A. Carr, Raymond J. Deshaies

    Research output: Contribution to journalArticle

    125 Citations (Scopus)

    Abstract

    Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 early anaphase release ( FEAR) network initiates dispersal of active Cdc14 throughout the cell. We report that the FEAR network promotes phosphorylation of Net1 by cyclin-dependent kinase (Cdk) complexed with cyclin B1 or cyclin B2. These phosphorylations appear to be required for FEAR and sustain the proper timing of late mitotic events. Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis.

    Original languageEnglish
    Pages (from-to)516-519
    Number of pages4
    JournalScience
    Volume305
    Issue number5683
    DOIs
    Publication statusPublished - 2004

    Cite this

    Azzam, R., Chen, S. L., Shou, W., Mah, A. S., Alexandru, G., Nasmyth, K., ... Deshaies, R. J. (2004). Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus. Science, 305(5683), 516-519. https://doi.org/10.1126/science.1099402
    Azzam, Ramzi ; Chen, Susan L. ; Shou, Wenying ; Mah, Angie S. ; Alexandru, Gabriela ; Nasmyth, Kim ; Annan, Roland S. ; Carr, Steven A. ; Deshaies, Raymond J. / Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus. In: Science. 2004 ; Vol. 305, No. 5683. pp. 516-519.
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    title = "Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus",
    abstract = "Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 early anaphase release ( FEAR) network initiates dispersal of active Cdc14 throughout the cell. We report that the FEAR network promotes phosphorylation of Net1 by cyclin-dependent kinase (Cdk) complexed with cyclin B1 or cyclin B2. These phosphorylations appear to be required for FEAR and sustain the proper timing of late mitotic events. Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis.",
    author = "Ramzi Azzam and Chen, {Susan L.} and Wenying Shou and Mah, {Angie S.} and Gabriela Alexandru and Kim Nasmyth and Annan, {Roland S.} and Carr, {Steven A.} and Deshaies, {Raymond J.}",
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    Azzam, R, Chen, SL, Shou, W, Mah, AS, Alexandru, G, Nasmyth, K, Annan, RS, Carr, SA & Deshaies, RJ 2004, 'Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus', Science, vol. 305, no. 5683, pp. 516-519. https://doi.org/10.1126/science.1099402

    Phosphorylation by cyclin B-Cdk underlies release of mitotic exit activator Cdc14 from the nucleolus. / Azzam, Ramzi; Chen, Susan L.; Shou, Wenying; Mah, Angie S.; Alexandru, Gabriela; Nasmyth, Kim; Annan, Roland S.; Carr, Steven A.; Deshaies, Raymond J.

    In: Science, Vol. 305, No. 5683, 2004, p. 516-519.

    Research output: Contribution to journalArticle

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    AU - Azzam, Ramzi

    AU - Chen, Susan L.

    AU - Shou, Wenying

    AU - Mah, Angie S.

    AU - Alexandru, Gabriela

    AU - Nasmyth, Kim

    AU - Annan, Roland S.

    AU - Carr, Steven A.

    AU - Deshaies, Raymond J.

    PY - 2004

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    AB - Budding yeast protein phosphatase Cdc14 is sequestered in the nucleolus in an inactive state during interphase by the anchor protein Net1. Upon entry into anaphase, the Cdc14 early anaphase release ( FEAR) network initiates dispersal of active Cdc14 throughout the cell. We report that the FEAR network promotes phosphorylation of Net1 by cyclin-dependent kinase (Cdk) complexed with cyclin B1 or cyclin B2. These phosphorylations appear to be required for FEAR and sustain the proper timing of late mitotic events. Thus, a regulatory circuit exists to ensure that the arbiter of the mitotic state, Cdk, sets in motion events that culminate in exit from mitosis.

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