Resistance of Galactoside-Terminated Alkanethiol Self-Assembled Monolayers to Marine Fouling Organisms

Thomas Ederth, Tobias Ekblad, Michala E. Pettitt, Sheelagh L. Conlan, Chun-Xia Du, Maureen E. Callow, James A. Callow, Robert Mutton, Anthony S. Clare, Fraddry D'Souza, Glen Donnelly, Anouk Bruin, Peter R. Willemsen, Xueju J. Su, Su Wang, Qi Zhao, Markus Hederos, Peter Konradsson, Bo Liedberg

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    Self-assembled monolayers (SAMs) of galactoside-terminated alkanethiols have protein-resistance properties which can be tuned via the degree of methylation [Langmuir 2005, 21, 2971-2980]. Specifically, a partially methylated compound was more resistant to nonspecific protein adsorption than the hydroxylated or fully methylated counterparts. We investigate whether this also holds true for resistance to the attachment and adhesion of a range of marine species, in order to clarify to what extent resistance to protein adsorption correlates with the more complex adhesion of fouling organisms. The partially methylated galactoside-terminated SAM was further compared to a mixed monolayer of omega-substituted methyl- and hydroxyl-terminated alkanethiols with wetting properties and surface ratio of hydroxyl to methyl groups matching that of the galactoside. The settlement (initial attachment) and adhesion strength of four model marine fouling organisms were investigated, representing both micro- and macrofoulers; two bacteria (Cobetia marina and Marinobacter hydrocarbonoclasticus), barnacle cypris larvae (Balanus amphitrite), and algal zoospores (Ulva linza). The minimum in protein adsorption onto the partially methylated galactoside surface was partly reproduced in the marine fouling assays, providing some support for a relationship between protein resistance and adhesion of marine fouling organisms. The mixed alkanethiol SAM, which was matched in wettability to the partially methylated galactoside SAM, consistently showed higher settlement (initial attachment) of test organisms than the galactoside, implying that both wettability and surface chemistry are insufficient to explain differences in fouling resistance. We suggest that differences in the structure of interfacial water may explain the variation in adhesion to these SAMs.

    Original languageEnglish
    Pages (from-to)3890-3901
    Number of pages12
    JournalACS Applied Materials & Interfaces
    Volume3
    Issue number10
    DOIs
    Publication statusPublished - Oct 2011

    Keywords

    • self-assembled monolayer
    • marine biofouling
    • Cobetia marina
    • Marinobacter hydrocarbonoclasticus
    • Balanus amphitrite
    • Ulva linza
    • BALANUS-AMPHITRITE DARWIN
    • GREEN-ALGA ENTEROMORPHA
    • SURFACE FREE-ENERGY
    • PROTEIN ADSORPTION
    • ADHESION STRENGTH
    • ULVA-LINZA
    • POLY(ETHYLENE GLYCOL)
    • OLIGO(ETHYLENE OXIDE)
    • WETTING PROPERTIES
    • SETTLED SPORES

    Cite this

    Ederth, T., Ekblad, T., Pettitt, M. E., Conlan, S. L., Du, C-X., Callow, M. E., ... Liedberg, B. (2011). Resistance of Galactoside-Terminated Alkanethiol Self-Assembled Monolayers to Marine Fouling Organisms. ACS Applied Materials & Interfaces, 3(10), 3890-3901. https://doi.org/10.1021/am200726a
    Ederth, Thomas ; Ekblad, Tobias ; Pettitt, Michala E. ; Conlan, Sheelagh L. ; Du, Chun-Xia ; Callow, Maureen E. ; Callow, James A. ; Mutton, Robert ; Clare, Anthony S. ; D'Souza, Fraddry ; Donnelly, Glen ; Bruin, Anouk ; Willemsen, Peter R. ; Su, Xueju J. ; Wang, Su ; Zhao, Qi ; Hederos, Markus ; Konradsson, Peter ; Liedberg, Bo. / Resistance of Galactoside-Terminated Alkanethiol Self-Assembled Monolayers to Marine Fouling Organisms. In: ACS Applied Materials & Interfaces. 2011 ; Vol. 3, No. 10. pp. 3890-3901.
    @article{ca820a6fd8144f828ac5c0ef810ba4d8,
    title = "Resistance of Galactoside-Terminated Alkanethiol Self-Assembled Monolayers to Marine Fouling Organisms",
    abstract = "Self-assembled monolayers (SAMs) of galactoside-terminated alkanethiols have protein-resistance properties which can be tuned via the degree of methylation [Langmuir 2005, 21, 2971-2980]. Specifically, a partially methylated compound was more resistant to nonspecific protein adsorption than the hydroxylated or fully methylated counterparts. We investigate whether this also holds true for resistance to the attachment and adhesion of a range of marine species, in order to clarify to what extent resistance to protein adsorption correlates with the more complex adhesion of fouling organisms. The partially methylated galactoside-terminated SAM was further compared to a mixed monolayer of omega-substituted methyl- and hydroxyl-terminated alkanethiols with wetting properties and surface ratio of hydroxyl to methyl groups matching that of the galactoside. The settlement (initial attachment) and adhesion strength of four model marine fouling organisms were investigated, representing both micro- and macrofoulers; two bacteria (Cobetia marina and Marinobacter hydrocarbonoclasticus), barnacle cypris larvae (Balanus amphitrite), and algal zoospores (Ulva linza). The minimum in protein adsorption onto the partially methylated galactoside surface was partly reproduced in the marine fouling assays, providing some support for a relationship between protein resistance and adhesion of marine fouling organisms. The mixed alkanethiol SAM, which was matched in wettability to the partially methylated galactoside SAM, consistently showed higher settlement (initial attachment) of test organisms than the galactoside, implying that both wettability and surface chemistry are insufficient to explain differences in fouling resistance. We suggest that differences in the structure of interfacial water may explain the variation in adhesion to these SAMs.",
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    author = "Thomas Ederth and Tobias Ekblad and Pettitt, {Michala E.} and Conlan, {Sheelagh L.} and Chun-Xia Du and Callow, {Maureen E.} and Callow, {James A.} and Robert Mutton and Clare, {Anthony S.} and Fraddry D'Souza and Glen Donnelly and Anouk Bruin and Willemsen, {Peter R.} and Su, {Xueju J.} and Su Wang and Qi Zhao and Markus Hederos and Peter Konradsson and Bo Liedberg",
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    language = "English",
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    }

    Ederth, T, Ekblad, T, Pettitt, ME, Conlan, SL, Du, C-X, Callow, ME, Callow, JA, Mutton, R, Clare, AS, D'Souza, F, Donnelly, G, Bruin, A, Willemsen, PR, Su, XJ, Wang, S, Zhao, Q, Hederos, M, Konradsson, P & Liedberg, B 2011, 'Resistance of Galactoside-Terminated Alkanethiol Self-Assembled Monolayers to Marine Fouling Organisms', ACS Applied Materials & Interfaces, vol. 3, no. 10, pp. 3890-3901. https://doi.org/10.1021/am200726a

    Resistance of Galactoside-Terminated Alkanethiol Self-Assembled Monolayers to Marine Fouling Organisms. / Ederth, Thomas; Ekblad, Tobias; Pettitt, Michala E.; Conlan, Sheelagh L.; Du, Chun-Xia; Callow, Maureen E.; Callow, James A.; Mutton, Robert; Clare, Anthony S.; D'Souza, Fraddry; Donnelly, Glen; Bruin, Anouk; Willemsen, Peter R.; Su, Xueju J.; Wang, Su; Zhao, Qi; Hederos, Markus; Konradsson, Peter; Liedberg, Bo.

    In: ACS Applied Materials & Interfaces, Vol. 3, No. 10, 10.2011, p. 3890-3901.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Resistance of Galactoside-Terminated Alkanethiol Self-Assembled Monolayers to Marine Fouling Organisms

    AU - Ederth, Thomas

    AU - Ekblad, Tobias

    AU - Pettitt, Michala E.

    AU - Conlan, Sheelagh L.

    AU - Du, Chun-Xia

    AU - Callow, Maureen E.

    AU - Callow, James A.

    AU - Mutton, Robert

    AU - Clare, Anthony S.

    AU - D'Souza, Fraddry

    AU - Donnelly, Glen

    AU - Bruin, Anouk

    AU - Willemsen, Peter R.

    AU - Su, Xueju J.

    AU - Wang, Su

    AU - Zhao, Qi

    AU - Hederos, Markus

    AU - Konradsson, Peter

    AU - Liedberg, Bo

    PY - 2011/10

    Y1 - 2011/10

    N2 - Self-assembled monolayers (SAMs) of galactoside-terminated alkanethiols have protein-resistance properties which can be tuned via the degree of methylation [Langmuir 2005, 21, 2971-2980]. Specifically, a partially methylated compound was more resistant to nonspecific protein adsorption than the hydroxylated or fully methylated counterparts. We investigate whether this also holds true for resistance to the attachment and adhesion of a range of marine species, in order to clarify to what extent resistance to protein adsorption correlates with the more complex adhesion of fouling organisms. The partially methylated galactoside-terminated SAM was further compared to a mixed monolayer of omega-substituted methyl- and hydroxyl-terminated alkanethiols with wetting properties and surface ratio of hydroxyl to methyl groups matching that of the galactoside. The settlement (initial attachment) and adhesion strength of four model marine fouling organisms were investigated, representing both micro- and macrofoulers; two bacteria (Cobetia marina and Marinobacter hydrocarbonoclasticus), barnacle cypris larvae (Balanus amphitrite), and algal zoospores (Ulva linza). The minimum in protein adsorption onto the partially methylated galactoside surface was partly reproduced in the marine fouling assays, providing some support for a relationship between protein resistance and adhesion of marine fouling organisms. The mixed alkanethiol SAM, which was matched in wettability to the partially methylated galactoside SAM, consistently showed higher settlement (initial attachment) of test organisms than the galactoside, implying that both wettability and surface chemistry are insufficient to explain differences in fouling resistance. We suggest that differences in the structure of interfacial water may explain the variation in adhesion to these SAMs.

    AB - Self-assembled monolayers (SAMs) of galactoside-terminated alkanethiols have protein-resistance properties which can be tuned via the degree of methylation [Langmuir 2005, 21, 2971-2980]. Specifically, a partially methylated compound was more resistant to nonspecific protein adsorption than the hydroxylated or fully methylated counterparts. We investigate whether this also holds true for resistance to the attachment and adhesion of a range of marine species, in order to clarify to what extent resistance to protein adsorption correlates with the more complex adhesion of fouling organisms. The partially methylated galactoside-terminated SAM was further compared to a mixed monolayer of omega-substituted methyl- and hydroxyl-terminated alkanethiols with wetting properties and surface ratio of hydroxyl to methyl groups matching that of the galactoside. The settlement (initial attachment) and adhesion strength of four model marine fouling organisms were investigated, representing both micro- and macrofoulers; two bacteria (Cobetia marina and Marinobacter hydrocarbonoclasticus), barnacle cypris larvae (Balanus amphitrite), and algal zoospores (Ulva linza). The minimum in protein adsorption onto the partially methylated galactoside surface was partly reproduced in the marine fouling assays, providing some support for a relationship between protein resistance and adhesion of marine fouling organisms. The mixed alkanethiol SAM, which was matched in wettability to the partially methylated galactoside SAM, consistently showed higher settlement (initial attachment) of test organisms than the galactoside, implying that both wettability and surface chemistry are insufficient to explain differences in fouling resistance. We suggest that differences in the structure of interfacial water may explain the variation in adhesion to these SAMs.

    KW - self-assembled monolayer

    KW - marine biofouling

    KW - Cobetia marina

    KW - Marinobacter hydrocarbonoclasticus

    KW - Balanus amphitrite

    KW - Ulva linza

    KW - BALANUS-AMPHITRITE DARWIN

    KW - GREEN-ALGA ENTEROMORPHA

    KW - SURFACE FREE-ENERGY

    KW - PROTEIN ADSORPTION

    KW - ADHESION STRENGTH

    KW - ULVA-LINZA

    KW - POLY(ETHYLENE GLYCOL)

    KW - OLIGO(ETHYLENE OXIDE)

    KW - WETTING PROPERTIES

    KW - SETTLED SPORES

    U2 - 10.1021/am200726a

    DO - 10.1021/am200726a

    M3 - Article

    C2 - 21916438

    VL - 3

    SP - 3890

    EP - 3901

    JO - ACS Applied Materials & Interfaces

    JF - ACS Applied Materials & Interfaces

    SN - 1944-8244

    IS - 10

    ER -