Resistance to Rhynchosporium commune in a collection of European spring barley germplasm

Mark E. Looseley (Lead / Corresponding author), Lucie L. Griffe, Bianca Büttner, Kathryn M. Wright, Jill Middlefell-Williams, Hazel Bull, Paul D. Shaw, Malcolm Macaulay, Allan Booth, Günther Schweizer, Joanne R. Russell, Robbie Waugh, William T. B. Thomas, Anna Avrova

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Rhynchosporium commune is a fungal pathogen of barley which causes a highly destructive and economically important disease known as rhynchosporium. Genome-wide association mapping was used to investigate the genetic control of host resistance to R. commune in a collection of predominantly European spring barley accessions. Multi-year disease nursery field trials revealed 8 significant resistance quantitative trait loci (QTL), whilst a separate association mapping analysis using historical data from UK national and recommended list trials identified 9 significant associations. The most significant association identified in both current and historical data sources, collocated with the known position of the major resistance gene Rrs1. Seedling assays with R. commune single-spore isolates expressing the corresponding avirulence protein NIP1 confirmed that this locus is Rrs1. These results highlight the significant and continuing contribution of Rrs1 to host resistance in current elite spring barley germplasm. Varietal height was shown to be negatively correlated with disease severity, and a resistance QTL was identified that co-localised with the semi-dwarfing gene sdw1, previously shown to contribute to disease escape. The remaining QTL represent novel resistances that are present within European spring barley accessions. Associated markers to Rrs1 and other resistance loci, identified in this study, represent a set of tools that can be exploited by breeders for the sustainable deployment of varietal resistance in new cultivars.

Original languageEnglish
Pages (from-to)2513-2528
Number of pages16
JournalTheoretical and Applied Genetics
Volume131
Issue number12
Early online date27 Aug 2018
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

Rhynchosporium
spring barley
Hordeum
Quantitative Trait Loci
quantitative trait loci
germplasm
chromosome mapping
varietal resistance
loci
dwarfing
genetic resistance
disease severity
Nurseries
Information Storage and Retrieval
field experimentation
genes
spores
Spores
Seedlings
barley

Keywords

  • Ascomycota/pathogenicity
  • Chromosome Mapping
  • Disease Resistance/genetics
  • Genetic Association Studies
  • Genetic Markers
  • Genotype
  • Hordeum/genetics
  • Phenotype
  • Plant Diseases/genetics
  • Polymorphism, Single Nucleotide
  • Quantitative Trait Loci

Cite this

Looseley, M. E., Griffe, L. L., Büttner, B., Wright, K. M., Middlefell-Williams, J., Bull, H., ... Avrova, A. (2018). Resistance to Rhynchosporium commune in a collection of European spring barley germplasm. Theoretical and Applied Genetics, 131(12), 2513-2528. https://doi.org/10.1007/s00122-018-3168-5
Looseley, Mark E. ; Griffe, Lucie L. ; Büttner, Bianca ; Wright, Kathryn M. ; Middlefell-Williams, Jill ; Bull, Hazel ; Shaw, Paul D. ; Macaulay, Malcolm ; Booth, Allan ; Schweizer, Günther ; Russell, Joanne R. ; Waugh, Robbie ; Thomas, William T. B. ; Avrova, Anna. / Resistance to Rhynchosporium commune in a collection of European spring barley germplasm. In: Theoretical and Applied Genetics. 2018 ; Vol. 131, No. 12. pp. 2513-2528.
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abstract = "Rhynchosporium commune is a fungal pathogen of barley which causes a highly destructive and economically important disease known as rhynchosporium. Genome-wide association mapping was used to investigate the genetic control of host resistance to R. commune in a collection of predominantly European spring barley accessions. Multi-year disease nursery field trials revealed 8 significant resistance quantitative trait loci (QTL), whilst a separate association mapping analysis using historical data from UK national and recommended list trials identified 9 significant associations. The most significant association identified in both current and historical data sources, collocated with the known position of the major resistance gene Rrs1. Seedling assays with R. commune single-spore isolates expressing the corresponding avirulence protein NIP1 confirmed that this locus is Rrs1. These results highlight the significant and continuing contribution of Rrs1 to host resistance in current elite spring barley germplasm. Varietal height was shown to be negatively correlated with disease severity, and a resistance QTL was identified that co-localised with the semi-dwarfing gene sdw1, previously shown to contribute to disease escape. The remaining QTL represent novel resistances that are present within European spring barley accessions. Associated markers to Rrs1 and other resistance loci, identified in this study, represent a set of tools that can be exploited by breeders for the sustainable deployment of varietal resistance in new cultivars.",
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Looseley, ME, Griffe, LL, Büttner, B, Wright, KM, Middlefell-Williams, J, Bull, H, Shaw, PD, Macaulay, M, Booth, A, Schweizer, G, Russell, JR, Waugh, R, Thomas, WTB & Avrova, A 2018, 'Resistance to Rhynchosporium commune in a collection of European spring barley germplasm', Theoretical and Applied Genetics, vol. 131, no. 12, pp. 2513-2528. https://doi.org/10.1007/s00122-018-3168-5

Resistance to Rhynchosporium commune in a collection of European spring barley germplasm. / Looseley, Mark E. (Lead / Corresponding author); Griffe, Lucie L.; Büttner, Bianca; Wright, Kathryn M.; Middlefell-Williams, Jill; Bull, Hazel; Shaw, Paul D.; Macaulay, Malcolm; Booth, Allan; Schweizer, Günther; Russell, Joanne R.; Waugh, Robbie; Thomas, William T. B.; Avrova, Anna.

In: Theoretical and Applied Genetics, Vol. 131, No. 12, 01.12.2018, p. 2513-2528.

Research output: Contribution to journalArticle

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T1 - Resistance to Rhynchosporium commune in a collection of European spring barley germplasm

AU - Looseley, Mark E.

AU - Griffe, Lucie L.

AU - Büttner, Bianca

AU - Wright, Kathryn M.

AU - Middlefell-Williams, Jill

AU - Bull, Hazel

AU - Shaw, Paul D.

AU - Macaulay, Malcolm

AU - Booth, Allan

AU - Schweizer, Günther

AU - Russell, Joanne R.

AU - Waugh, Robbie

AU - Thomas, William T. B.

AU - Avrova, Anna

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Rhynchosporium commune is a fungal pathogen of barley which causes a highly destructive and economically important disease known as rhynchosporium. Genome-wide association mapping was used to investigate the genetic control of host resistance to R. commune in a collection of predominantly European spring barley accessions. Multi-year disease nursery field trials revealed 8 significant resistance quantitative trait loci (QTL), whilst a separate association mapping analysis using historical data from UK national and recommended list trials identified 9 significant associations. The most significant association identified in both current and historical data sources, collocated with the known position of the major resistance gene Rrs1. Seedling assays with R. commune single-spore isolates expressing the corresponding avirulence protein NIP1 confirmed that this locus is Rrs1. These results highlight the significant and continuing contribution of Rrs1 to host resistance in current elite spring barley germplasm. Varietal height was shown to be negatively correlated with disease severity, and a resistance QTL was identified that co-localised with the semi-dwarfing gene sdw1, previously shown to contribute to disease escape. The remaining QTL represent novel resistances that are present within European spring barley accessions. Associated markers to Rrs1 and other resistance loci, identified in this study, represent a set of tools that can be exploited by breeders for the sustainable deployment of varietal resistance in new cultivars.

AB - Rhynchosporium commune is a fungal pathogen of barley which causes a highly destructive and economically important disease known as rhynchosporium. Genome-wide association mapping was used to investigate the genetic control of host resistance to R. commune in a collection of predominantly European spring barley accessions. Multi-year disease nursery field trials revealed 8 significant resistance quantitative trait loci (QTL), whilst a separate association mapping analysis using historical data from UK national and recommended list trials identified 9 significant associations. The most significant association identified in both current and historical data sources, collocated with the known position of the major resistance gene Rrs1. Seedling assays with R. commune single-spore isolates expressing the corresponding avirulence protein NIP1 confirmed that this locus is Rrs1. These results highlight the significant and continuing contribution of Rrs1 to host resistance in current elite spring barley germplasm. Varietal height was shown to be negatively correlated with disease severity, and a resistance QTL was identified that co-localised with the semi-dwarfing gene sdw1, previously shown to contribute to disease escape. The remaining QTL represent novel resistances that are present within European spring barley accessions. Associated markers to Rrs1 and other resistance loci, identified in this study, represent a set of tools that can be exploited by breeders for the sustainable deployment of varietal resistance in new cultivars.

KW - Ascomycota/pathogenicity

KW - Chromosome Mapping

KW - Disease Resistance/genetics

KW - Genetic Association Studies

KW - Genetic Markers

KW - Genotype

KW - Hordeum/genetics

KW - Phenotype

KW - Plant Diseases/genetics

KW - Polymorphism, Single Nucleotide

KW - Quantitative Trait Loci

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DO - 10.1007/s00122-018-3168-5

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VL - 131

SP - 2513

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JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

SN - 0040-5752

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Looseley ME, Griffe LL, Büttner B, Wright KM, Middlefell-Williams J, Bull H et al. Resistance to Rhynchosporium commune in a collection of European spring barley germplasm. Theoretical and Applied Genetics. 2018 Dec 1;131(12):2513-2528. https://doi.org/10.1007/s00122-018-3168-5