RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor

Kostya I. Panov, Tatiana B. Panova, Olivier Gadal, Kaori Nishiyama, Takashi Saito, Jackie Russell, Joost C. B. M. Zomerdijk

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Eukaryotic RNA polymerases are large complexes, 12 subunits of which are structurally or functionally homologous across the three polymerase classes. Each class has a set of specific subunits, likely targets of their cognate transcription factors. We have identified and characterized a human RNA polymerase I (Poll I)-specific subunit, previously identified as ASE-1 (antisense of ERCC1) and as CD3 epsilon-associated signal transducer (CAST), and here termed CAST or human Pol I-associated factor of 49 kDa (hPAF49), after mouse orthologue PAF49. We provide evidence for growth-regulated Tyr phosphorylation of CAST/hPAF49, specifically in initiation-competent Pol I[3 complexes in HeLa cells, at a conserved residue also known to be important for signaling during T-cell activation. CAST/hPAF49 can interact with activator upstream binding factor (UBF) and, weakly, with selectivity factor 1 (SL1) at the rDNA (ribosomal DNA repeat sequence encoding the 18S, 5.8S, and 28S rRNA genes) promoter. CAST/hPAF49-specific antibodies and excess CAST/hPAF49 protein, which have no effect on basal Pol I transcription, inhibit UBF-activated transcription following functional SLl-Pol I-rDNA complex assembly and disrupt the interaction of UBF with CAST/hPAF49, suggesting that interaction of this Pol I-specific subunit with UBF is crucial for activation. Drawing on parallels between mammalian and Saccharomyces cerevisiae Pol I transcription machineries, we advance one model for CAST/hPAF49 function in which the network of interactions of Pol I-specific subunits with UBF facilitates conformational changes of the polymerase, leading to stabilization of the Poll I-template complex and, thereby, activation of transcription.

    Original languageEnglish
    Pages (from-to)5436-5448
    Number of pages13
    JournalMolecular and Cellular Biology
    Volume26
    Issue number14
    DOIs
    Publication statusPublished - Jul 2006

    Fingerprint

    RNA Polymerase I
    Transducers
    Transcriptional Activation
    Ribosomal DNA
    transcription factor UBF
    DNA-Directed RNA Polymerases
    HeLa Cells
    rRNA Genes
    Genetic Promoter Regions
    Saccharomyces cerevisiae
    Transcription Factors
    Phosphorylation
    T-Lymphocytes
    Antibodies

    Cite this

    Panov, Kostya I. ; Panova, Tatiana B. ; Gadal, Olivier ; Nishiyama, Kaori ; Saito, Takashi ; Russell, Jackie ; Zomerdijk, Joost C. B. M. / RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor. In: Molecular and Cellular Biology. 2006 ; Vol. 26, No. 14. pp. 5436-5448.
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    title = "RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor",
    abstract = "Eukaryotic RNA polymerases are large complexes, 12 subunits of which are structurally or functionally homologous across the three polymerase classes. Each class has a set of specific subunits, likely targets of their cognate transcription factors. We have identified and characterized a human RNA polymerase I (Poll I)-specific subunit, previously identified as ASE-1 (antisense of ERCC1) and as CD3 epsilon-associated signal transducer (CAST), and here termed CAST or human Pol I-associated factor of 49 kDa (hPAF49), after mouse orthologue PAF49. We provide evidence for growth-regulated Tyr phosphorylation of CAST/hPAF49, specifically in initiation-competent Pol I[3 complexes in HeLa cells, at a conserved residue also known to be important for signaling during T-cell activation. CAST/hPAF49 can interact with activator upstream binding factor (UBF) and, weakly, with selectivity factor 1 (SL1) at the rDNA (ribosomal DNA repeat sequence encoding the 18S, 5.8S, and 28S rRNA genes) promoter. CAST/hPAF49-specific antibodies and excess CAST/hPAF49 protein, which have no effect on basal Pol I transcription, inhibit UBF-activated transcription following functional SLl-Pol I-rDNA complex assembly and disrupt the interaction of UBF with CAST/hPAF49, suggesting that interaction of this Pol I-specific subunit with UBF is crucial for activation. Drawing on parallels between mammalian and Saccharomyces cerevisiae Pol I transcription machineries, we advance one model for CAST/hPAF49 function in which the network of interactions of Pol I-specific subunits with UBF facilitates conformational changes of the polymerase, leading to stabilization of the Poll I-template complex and, thereby, activation of transcription.",
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    year = "2006",
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    RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor. / Panov, Kostya I.; Panova, Tatiana B.; Gadal, Olivier; Nishiyama, Kaori; Saito, Takashi; Russell, Jackie; Zomerdijk, Joost C. B. M.

    In: Molecular and Cellular Biology, Vol. 26, No. 14, 07.2006, p. 5436-5448.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor

    AU - Panov, Kostya I.

    AU - Panova, Tatiana B.

    AU - Gadal, Olivier

    AU - Nishiyama, Kaori

    AU - Saito, Takashi

    AU - Russell, Jackie

    AU - Zomerdijk, Joost C. B. M.

    PY - 2006/7

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    N2 - Eukaryotic RNA polymerases are large complexes, 12 subunits of which are structurally or functionally homologous across the three polymerase classes. Each class has a set of specific subunits, likely targets of their cognate transcription factors. We have identified and characterized a human RNA polymerase I (Poll I)-specific subunit, previously identified as ASE-1 (antisense of ERCC1) and as CD3 epsilon-associated signal transducer (CAST), and here termed CAST or human Pol I-associated factor of 49 kDa (hPAF49), after mouse orthologue PAF49. We provide evidence for growth-regulated Tyr phosphorylation of CAST/hPAF49, specifically in initiation-competent Pol I[3 complexes in HeLa cells, at a conserved residue also known to be important for signaling during T-cell activation. CAST/hPAF49 can interact with activator upstream binding factor (UBF) and, weakly, with selectivity factor 1 (SL1) at the rDNA (ribosomal DNA repeat sequence encoding the 18S, 5.8S, and 28S rRNA genes) promoter. CAST/hPAF49-specific antibodies and excess CAST/hPAF49 protein, which have no effect on basal Pol I transcription, inhibit UBF-activated transcription following functional SLl-Pol I-rDNA complex assembly and disrupt the interaction of UBF with CAST/hPAF49, suggesting that interaction of this Pol I-specific subunit with UBF is crucial for activation. Drawing on parallels between mammalian and Saccharomyces cerevisiae Pol I transcription machineries, we advance one model for CAST/hPAF49 function in which the network of interactions of Pol I-specific subunits with UBF facilitates conformational changes of the polymerase, leading to stabilization of the Poll I-template complex and, thereby, activation of transcription.

    AB - Eukaryotic RNA polymerases are large complexes, 12 subunits of which are structurally or functionally homologous across the three polymerase classes. Each class has a set of specific subunits, likely targets of their cognate transcription factors. We have identified and characterized a human RNA polymerase I (Poll I)-specific subunit, previously identified as ASE-1 (antisense of ERCC1) and as CD3 epsilon-associated signal transducer (CAST), and here termed CAST or human Pol I-associated factor of 49 kDa (hPAF49), after mouse orthologue PAF49. We provide evidence for growth-regulated Tyr phosphorylation of CAST/hPAF49, specifically in initiation-competent Pol I[3 complexes in HeLa cells, at a conserved residue also known to be important for signaling during T-cell activation. CAST/hPAF49 can interact with activator upstream binding factor (UBF) and, weakly, with selectivity factor 1 (SL1) at the rDNA (ribosomal DNA repeat sequence encoding the 18S, 5.8S, and 28S rRNA genes) promoter. CAST/hPAF49-specific antibodies and excess CAST/hPAF49 protein, which have no effect on basal Pol I transcription, inhibit UBF-activated transcription following functional SLl-Pol I-rDNA complex assembly and disrupt the interaction of UBF with CAST/hPAF49, suggesting that interaction of this Pol I-specific subunit with UBF is crucial for activation. Drawing on parallels between mammalian and Saccharomyces cerevisiae Pol I transcription machineries, we advance one model for CAST/hPAF49 function in which the network of interactions of Pol I-specific subunits with UBF facilitates conformational changes of the polymerase, leading to stabilization of the Poll I-template complex and, thereby, activation of transcription.

    U2 - 10.1128/MCB.00230-06

    DO - 10.1128/MCB.00230-06

    M3 - Article

    VL - 26

    SP - 5436

    EP - 5448

    JO - Molecular and Cellular Biology

    JF - Molecular and Cellular Biology

    SN - 0270-7306

    IS - 14

    ER -