Semen characteristics in HIV-1 positive men and the effect of semen washing

Ali S Lasheeb, Julie King, Jonathan K Ball, Rebecca Curran, Christopher L R Barratt, Masoud Afnan, Deenan Pillay

Research output: Contribution to journalArticle

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Abstract

We have undertaken an analysis of semen from HIV infected men with regard to sperm counts and motility, non-spermatozoal cells, and viral nucleic acid. Regression analysis showed that sperm concentration and motility were positively associated with blood CD4 cell count. By contrast, non-spermatozoal cell concentration (round cells) was inversely related to CD4 count. Extracellular HIV RNA was detected in the majority of semen samples and proviral DNA in a minority. Percoll gradient washing of 12 semen samples yielded six samples containing adequate sperm concentration for analysis. This washing procedure reduced prewash extracellular RNA to below detectable limits in all cases; proviral DNA present in two of the six prewash samples was also reduced to below detectable limits after washing. We conclude that semen washing before artificial insemination may reduce the risk of HIV transmission from an infected man to an uninfected woman. However, further evidence from prospective analyses of such an approach is required.

Original languageEnglish
Pages (from-to)303-305
Number of pages3
JournalGenitourinary Medicine
Volume73
Issue number4
DOIs
Publication statusPublished - Aug 1997

Fingerprint

Semen
HIV-1
Sperm Motility
HIV
CD4 Lymphocyte Count
RNA
Artificial Insemination
Semen Analysis
Sperm Count
Blood Cell Count
DNA
Nucleic Acids
Spermatozoa
Regression Analysis

Keywords

  • CD4 Lymphocyte Count
  • DNA, Viral/analysis
  • Feasibility Studies
  • HIV Seropositivity/immunology
  • HIV-1/genetics
  • Humans
  • Insemination, Artificial, Homologous
  • Male
  • Pilot Projects
  • RNA, Viral/analysis
  • Semen/virology
  • Sperm Count
  • Sperm Motility
  • Therapeutic Irrigation

Cite this

Lasheeb, A. S., King, J., Ball, J. K., Curran, R., Barratt, C. L. R., Afnan, M., & Pillay, D. (1997). Semen characteristics in HIV-1 positive men and the effect of semen washing. Genitourinary Medicine, 73(4), 303-305. https://doi.org/10.1136/sti.73.4.303
Lasheeb, Ali S ; King, Julie ; Ball, Jonathan K ; Curran, Rebecca ; Barratt, Christopher L R ; Afnan, Masoud ; Pillay, Deenan. / Semen characteristics in HIV-1 positive men and the effect of semen washing. In: Genitourinary Medicine. 1997 ; Vol. 73, No. 4. pp. 303-305.
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abstract = "We have undertaken an analysis of semen from HIV infected men with regard to sperm counts and motility, non-spermatozoal cells, and viral nucleic acid. Regression analysis showed that sperm concentration and motility were positively associated with blood CD4 cell count. By contrast, non-spermatozoal cell concentration (round cells) was inversely related to CD4 count. Extracellular HIV RNA was detected in the majority of semen samples and proviral DNA in a minority. Percoll gradient washing of 12 semen samples yielded six samples containing adequate sperm concentration for analysis. This washing procedure reduced prewash extracellular RNA to below detectable limits in all cases; proviral DNA present in two of the six prewash samples was also reduced to below detectable limits after washing. We conclude that semen washing before artificial insemination may reduce the risk of HIV transmission from an infected man to an uninfected woman. However, further evidence from prospective analyses of such an approach is required.",
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Lasheeb, AS, King, J, Ball, JK, Curran, R, Barratt, CLR, Afnan, M & Pillay, D 1997, 'Semen characteristics in HIV-1 positive men and the effect of semen washing', Genitourinary Medicine, vol. 73, no. 4, pp. 303-305. https://doi.org/10.1136/sti.73.4.303

Semen characteristics in HIV-1 positive men and the effect of semen washing. / Lasheeb, Ali S; King, Julie; Ball, Jonathan K; Curran, Rebecca; Barratt, Christopher L R; Afnan, Masoud; Pillay, Deenan.

In: Genitourinary Medicine, Vol. 73, No. 4, 08.1997, p. 303-305.

Research output: Contribution to journalArticle

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AU - King, Julie

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AU - Pillay, Deenan

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AB - We have undertaken an analysis of semen from HIV infected men with regard to sperm counts and motility, non-spermatozoal cells, and viral nucleic acid. Regression analysis showed that sperm concentration and motility were positively associated with blood CD4 cell count. By contrast, non-spermatozoal cell concentration (round cells) was inversely related to CD4 count. Extracellular HIV RNA was detected in the majority of semen samples and proviral DNA in a minority. Percoll gradient washing of 12 semen samples yielded six samples containing adequate sperm concentration for analysis. This washing procedure reduced prewash extracellular RNA to below detectable limits in all cases; proviral DNA present in two of the six prewash samples was also reduced to below detectable limits after washing. We conclude that semen washing before artificial insemination may reduce the risk of HIV transmission from an infected man to an uninfected woman. However, further evidence from prospective analyses of such an approach is required.

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