Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins

T Fontaine, Thierry Magnin, Angela Mehlert, Douglas Lamont, J P Latge, Michael A. J. Ferguson

    Research output: Contribution to journalArticle

    62 Citations (Scopus)

    Abstract

    Glycosylphosphatidylinositol (GPI)-anchored proteins have been identified in all eukaryotes. In fungi, structural and biosynthetic studies of GPIs have been restricted to the yeast Saccharomyces cerevisiae. In this article, four GPI-anchored proteins were purified from a membrane preparation of the human filamentous fungal pathogen Aspergillus fumigatus. Using new methodology applied to western blot protein bands, the GPI structures were characterized by ES-MS, fluorescence labeling, HPLC, and specific enzymatic digestions. The phosphatidylinositol moiety of the A. fumigatus GPI membrane anchors was shown to be an inositol-phosphoceramide containing mainly phytosphingosine and monohydroxylated C-24:0 fatty acid. In constrast to yeast, only ceramide was found in the GPI anchor structures of A. fumigatus, even for Gel1p, a homolog of Gas1p in S. cerevisiae that contains diacylglycerol. The A. fumigatus GPI glycan moiety is mainly a linear pentomannose structure linked to a glucosamine residue: Manalpha1-3Manalpha1-2Manalpha1-2Manalpha1-6Manalpha1-4GlcN.

    Original languageEnglish
    Pages (from-to)169-177
    Number of pages9
    JournalGlycobiology
    Volume13
    Issue number3
    DOIs
    Publication statusPublished - Mar 2003

    Cite this

    Fontaine, T ; Magnin, Thierry ; Mehlert, Angela ; Lamont, Douglas ; Latge, J P ; Ferguson, Michael A. J. . / Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins. In: Glycobiology. 2003 ; Vol. 13, No. 3. pp. 169-177.
    @article{d249d0918cb34131bbbb15577dd23275,
    title = "Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins",
    abstract = "Glycosylphosphatidylinositol (GPI)-anchored proteins have been identified in all eukaryotes. In fungi, structural and biosynthetic studies of GPIs have been restricted to the yeast Saccharomyces cerevisiae. In this article, four GPI-anchored proteins were purified from a membrane preparation of the human filamentous fungal pathogen Aspergillus fumigatus. Using new methodology applied to western blot protein bands, the GPI structures were characterized by ES-MS, fluorescence labeling, HPLC, and specific enzymatic digestions. The phosphatidylinositol moiety of the A. fumigatus GPI membrane anchors was shown to be an inositol-phosphoceramide containing mainly phytosphingosine and monohydroxylated C-24:0 fatty acid. In constrast to yeast, only ceramide was found in the GPI anchor structures of A. fumigatus, even for Gel1p, a homolog of Gas1p in S. cerevisiae that contains diacylglycerol. The A. fumigatus GPI glycan moiety is mainly a linear pentomannose structure linked to a glucosamine residue: Manalpha1-3Manalpha1-2Manalpha1-2Manalpha1-6Manalpha1-4GlcN.",
    author = "T Fontaine and Thierry Magnin and Angela Mehlert and Douglas Lamont and Latge, {J P} and Ferguson, {Michael A. J.}",
    year = "2003",
    month = "3",
    doi = "10.1093/glycob/cwg004",
    language = "English",
    volume = "13",
    pages = "169--177",
    journal = "Glycobiology",
    issn = "0959-6658",
    publisher = "Oxford University Press",
    number = "3",

    }

    Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins. / Fontaine, T ; Magnin, Thierry ; Mehlert, Angela; Lamont, Douglas ; Latge, J P ; Ferguson, Michael A. J. .

    In: Glycobiology, Vol. 13, No. 3, 03.2003, p. 169-177.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins

    AU - Fontaine, T

    AU - Magnin, Thierry

    AU - Mehlert, Angela

    AU - Lamont, Douglas

    AU - Latge, J P

    AU - Ferguson, Michael A. J.

    PY - 2003/3

    Y1 - 2003/3

    N2 - Glycosylphosphatidylinositol (GPI)-anchored proteins have been identified in all eukaryotes. In fungi, structural and biosynthetic studies of GPIs have been restricted to the yeast Saccharomyces cerevisiae. In this article, four GPI-anchored proteins were purified from a membrane preparation of the human filamentous fungal pathogen Aspergillus fumigatus. Using new methodology applied to western blot protein bands, the GPI structures were characterized by ES-MS, fluorescence labeling, HPLC, and specific enzymatic digestions. The phosphatidylinositol moiety of the A. fumigatus GPI membrane anchors was shown to be an inositol-phosphoceramide containing mainly phytosphingosine and monohydroxylated C-24:0 fatty acid. In constrast to yeast, only ceramide was found in the GPI anchor structures of A. fumigatus, even for Gel1p, a homolog of Gas1p in S. cerevisiae that contains diacylglycerol. The A. fumigatus GPI glycan moiety is mainly a linear pentomannose structure linked to a glucosamine residue: Manalpha1-3Manalpha1-2Manalpha1-2Manalpha1-6Manalpha1-4GlcN.

    AB - Glycosylphosphatidylinositol (GPI)-anchored proteins have been identified in all eukaryotes. In fungi, structural and biosynthetic studies of GPIs have been restricted to the yeast Saccharomyces cerevisiae. In this article, four GPI-anchored proteins were purified from a membrane preparation of the human filamentous fungal pathogen Aspergillus fumigatus. Using new methodology applied to western blot protein bands, the GPI structures were characterized by ES-MS, fluorescence labeling, HPLC, and specific enzymatic digestions. The phosphatidylinositol moiety of the A. fumigatus GPI membrane anchors was shown to be an inositol-phosphoceramide containing mainly phytosphingosine and monohydroxylated C-24:0 fatty acid. In constrast to yeast, only ceramide was found in the GPI anchor structures of A. fumigatus, even for Gel1p, a homolog of Gas1p in S. cerevisiae that contains diacylglycerol. The A. fumigatus GPI glycan moiety is mainly a linear pentomannose structure linked to a glucosamine residue: Manalpha1-3Manalpha1-2Manalpha1-2Manalpha1-6Manalpha1-4GlcN.

    U2 - 10.1093/glycob/cwg004

    DO - 10.1093/glycob/cwg004

    M3 - Article

    VL - 13

    SP - 169

    EP - 177

    JO - Glycobiology

    JF - Glycobiology

    SN - 0959-6658

    IS - 3

    ER -