The Hybrid Type Polyketide Synthase SteelyA Is Required for cAMP Signalling in Early Dictyostelium Development

Takaaki B. Narita, Zhi-Hui Chen, Pauline Schaap, Tamao Saito (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Background

    In our previous study we found that the expression of stlA showed peaks both in the early and last stages of development and that a product of SteelyA, 4-methyl-5-pentylbenzene-1,3-diol (MPBD), controlled Dictyostelium spore maturation during the latter. In this study we focused on the role of SteelyA in early stage development.
    Principal Findings

    Our stlA null mutant showed aggregation delay and abnormally small aggregation territories. Chemotaxis analysis revealed defective cAMP chemotaxis in the stlA null mutant. cAMP chemotaxis was restored by MPBD addition during early stage development. Assay for cAMP relay response revealed that the stlA null mutant had lower cAMP accumulation during aggregation, suggesting lower ACA activity than the wild type strain. Exogenous cAMP pulses rescued the aggregation defect of the stlA null strain in the absence of MPBD. Expression analysis of cAMP signalling genes revealed lower expression levels in the stlA null mutant during aggregation.
    Conclusion

    Our data indicate a regulatory function by SteelyA on cAMP signalling during aggregation and show that SteelyA is indispensable for full activation of ACA.
    Original languageEnglish
    Article numbere106634
    Number of pages9
    JournalPLoS ONE
    Volume9
    Issue number9
    DOIs
    Publication statusPublished - 15 Sep 2014

    Fingerprint

    Polyketide Synthases
    polyketide synthases
    Dictyostelium
    Chemotaxis
    glycols
    chemotaxis
    Agglomeration
    mutants
    Spores
    spores
    Genes
    4-methyl-5-pentylbenzene-1,3-diol
    Assays
    assays
    Chemical activation
    Defects
    genes

    Cite this

    @article{09232fabc7bd4845b002c118f6a9aad6,
    title = "The Hybrid Type Polyketide Synthase SteelyA Is Required for cAMP Signalling in Early Dictyostelium Development",
    abstract = "BackgroundIn our previous study we found that the expression of stlA showed peaks both in the early and last stages of development and that a product of SteelyA, 4-methyl-5-pentylbenzene-1,3-diol (MPBD), controlled Dictyostelium spore maturation during the latter. In this study we focused on the role of SteelyA in early stage development.Principal FindingsOur stlA null mutant showed aggregation delay and abnormally small aggregation territories. Chemotaxis analysis revealed defective cAMP chemotaxis in the stlA null mutant. cAMP chemotaxis was restored by MPBD addition during early stage development. Assay for cAMP relay response revealed that the stlA null mutant had lower cAMP accumulation during aggregation, suggesting lower ACA activity than the wild type strain. Exogenous cAMP pulses rescued the aggregation defect of the stlA null strain in the absence of MPBD. Expression analysis of cAMP signalling genes revealed lower expression levels in the stlA null mutant during aggregation.ConclusionOur data indicate a regulatory function by SteelyA on cAMP signalling during aggregation and show that SteelyA is indispensable for full activation of ACA.",
    author = "Narita, {Takaaki B.} and Zhi-Hui Chen and Pauline Schaap and Tamao Saito",
    year = "2014",
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    doi = "10.1371/journal.pone.0106634",
    language = "English",
    volume = "9",
    journal = "PLoS ONE",
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    publisher = "Public Library of Science",
    number = "9",

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    The Hybrid Type Polyketide Synthase SteelyA Is Required for cAMP Signalling in Early Dictyostelium Development. / Narita, Takaaki B.; Chen, Zhi-Hui; Schaap, Pauline; Saito, Tamao (Lead / Corresponding author).

    In: PLoS ONE, Vol. 9, No. 9, e106634, 15.09.2014.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - The Hybrid Type Polyketide Synthase SteelyA Is Required for cAMP Signalling in Early Dictyostelium Development

    AU - Narita, Takaaki B.

    AU - Chen, Zhi-Hui

    AU - Schaap, Pauline

    AU - Saito, Tamao

    PY - 2014/9/15

    Y1 - 2014/9/15

    N2 - BackgroundIn our previous study we found that the expression of stlA showed peaks both in the early and last stages of development and that a product of SteelyA, 4-methyl-5-pentylbenzene-1,3-diol (MPBD), controlled Dictyostelium spore maturation during the latter. In this study we focused on the role of SteelyA in early stage development.Principal FindingsOur stlA null mutant showed aggregation delay and abnormally small aggregation territories. Chemotaxis analysis revealed defective cAMP chemotaxis in the stlA null mutant. cAMP chemotaxis was restored by MPBD addition during early stage development. Assay for cAMP relay response revealed that the stlA null mutant had lower cAMP accumulation during aggregation, suggesting lower ACA activity than the wild type strain. Exogenous cAMP pulses rescued the aggregation defect of the stlA null strain in the absence of MPBD. Expression analysis of cAMP signalling genes revealed lower expression levels in the stlA null mutant during aggregation.ConclusionOur data indicate a regulatory function by SteelyA on cAMP signalling during aggregation and show that SteelyA is indispensable for full activation of ACA.

    AB - BackgroundIn our previous study we found that the expression of stlA showed peaks both in the early and last stages of development and that a product of SteelyA, 4-methyl-5-pentylbenzene-1,3-diol (MPBD), controlled Dictyostelium spore maturation during the latter. In this study we focused on the role of SteelyA in early stage development.Principal FindingsOur stlA null mutant showed aggregation delay and abnormally small aggregation territories. Chemotaxis analysis revealed defective cAMP chemotaxis in the stlA null mutant. cAMP chemotaxis was restored by MPBD addition during early stage development. Assay for cAMP relay response revealed that the stlA null mutant had lower cAMP accumulation during aggregation, suggesting lower ACA activity than the wild type strain. Exogenous cAMP pulses rescued the aggregation defect of the stlA null strain in the absence of MPBD. Expression analysis of cAMP signalling genes revealed lower expression levels in the stlA null mutant during aggregation.ConclusionOur data indicate a regulatory function by SteelyA on cAMP signalling during aggregation and show that SteelyA is indispensable for full activation of ACA.

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    DO - 10.1371/journal.pone.0106634

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    JO - PLoS ONE

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