The major glutathione S-transferase in salmonid fish livers is homologous to the mammalian pi-class GST

R J Dominey, I A Nimmo, A D Cronshaw, J D Hayes

    Research output: Contribution to journalArticle

    62 Citations (Scopus)

    Abstract

    1. The hepatic glutathione S-transferase (GST) isoenzymes were isolated and characterized from salmon, sea trout and rainbow trout. 2. In all three species the predominant GST expressed comprised subunits of Mr 24,800. These subunits each co-migrated with the rat pi-class Yf polypeptide during SDS/polyacrylamide gel electrophoresis. 3. Western blotting experiments demonstrated immunochemical cross-reactivity between the major salmonid and the rat pi-class GSTs. 4. The salmon GST of subunit Mr 24,800 was digested with cyanogen bromide and the peptides, once purified by reverse-phase HPLC, were subjected to automated amino acid sequencing. 5. Over the region sequenced, the salmon GST possessed about 65% homology with the rat and human pi-class GST.

    Original languageEnglish
    Pages (from-to)93-8
    Number of pages6
    JournalComparative Biochemistry and Physiology Part B: Comparative Biochemistry
    Volume100
    Issue number1
    Publication statusPublished - 1991

    Fingerprint

    Glutathione S-Transferase pi
    Glutathione Transferase
    Liver
    Fish
    Salmon
    Fishes
    Rats
    Cyanogen Bromide
    Peptides
    Trout
    Oncorhynchus mykiss
    Protein Sequence Analysis
    Oceans and Seas
    Isoenzymes
    Polyacrylamide Gel Electrophoresis
    Electrophoresis
    Western Blotting
    High Pressure Liquid Chromatography
    Amino Acids
    Experiments

    Keywords

    • Amino Acid Sequence
    • Animals
    • Glutathione Transferase/chemistry
    • Humans
    • Isoenzymes/chemistry
    • Liver/enzymology
    • Mammals
    • Molecular Sequence Data
    • Molecular Weight
    • Protein Conformation
    • Salmonidae/metabolism
    • Sequence Homology, Nucleic Acid
    • Species Specificity

    Cite this

    @article{3113164db2494996ba924bca5187bf08,
    title = "The major glutathione S-transferase in salmonid fish livers is homologous to the mammalian pi-class GST",
    abstract = "1. The hepatic glutathione S-transferase (GST) isoenzymes were isolated and characterized from salmon, sea trout and rainbow trout. 2. In all three species the predominant GST expressed comprised subunits of Mr 24,800. These subunits each co-migrated with the rat pi-class Yf polypeptide during SDS/polyacrylamide gel electrophoresis. 3. Western blotting experiments demonstrated immunochemical cross-reactivity between the major salmonid and the rat pi-class GSTs. 4. The salmon GST of subunit Mr 24,800 was digested with cyanogen bromide and the peptides, once purified by reverse-phase HPLC, were subjected to automated amino acid sequencing. 5. Over the region sequenced, the salmon GST possessed about 65{\%} homology with the rat and human pi-class GST.",
    keywords = "Amino Acid Sequence, Animals, Glutathione Transferase/chemistry, Humans, Isoenzymes/chemistry, Liver/enzymology, Mammals, Molecular Sequence Data, Molecular Weight, Protein Conformation, Salmonidae/metabolism, Sequence Homology, Nucleic Acid, Species Specificity",
    author = "Dominey, {R J} and Nimmo, {I A} and Cronshaw, {A D} and Hayes, {J D}",
    year = "1991",
    language = "English",
    volume = "100",
    pages = "93--8",
    journal = "Comparative Biochemistry and Physiology Part B: Comparative Biochemistry",
    issn = "0305-0491",
    publisher = "Elsevier",
    number = "1",

    }

    The major glutathione S-transferase in salmonid fish livers is homologous to the mammalian pi-class GST. / Dominey, R J; Nimmo, I A; Cronshaw, A D; Hayes, J D.

    In: Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, Vol. 100, No. 1, 1991, p. 93-8.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - The major glutathione S-transferase in salmonid fish livers is homologous to the mammalian pi-class GST

    AU - Dominey, R J

    AU - Nimmo, I A

    AU - Cronshaw, A D

    AU - Hayes, J D

    PY - 1991

    Y1 - 1991

    N2 - 1. The hepatic glutathione S-transferase (GST) isoenzymes were isolated and characterized from salmon, sea trout and rainbow trout. 2. In all three species the predominant GST expressed comprised subunits of Mr 24,800. These subunits each co-migrated with the rat pi-class Yf polypeptide during SDS/polyacrylamide gel electrophoresis. 3. Western blotting experiments demonstrated immunochemical cross-reactivity between the major salmonid and the rat pi-class GSTs. 4. The salmon GST of subunit Mr 24,800 was digested with cyanogen bromide and the peptides, once purified by reverse-phase HPLC, were subjected to automated amino acid sequencing. 5. Over the region sequenced, the salmon GST possessed about 65% homology with the rat and human pi-class GST.

    AB - 1. The hepatic glutathione S-transferase (GST) isoenzymes were isolated and characterized from salmon, sea trout and rainbow trout. 2. In all three species the predominant GST expressed comprised subunits of Mr 24,800. These subunits each co-migrated with the rat pi-class Yf polypeptide during SDS/polyacrylamide gel electrophoresis. 3. Western blotting experiments demonstrated immunochemical cross-reactivity between the major salmonid and the rat pi-class GSTs. 4. The salmon GST of subunit Mr 24,800 was digested with cyanogen bromide and the peptides, once purified by reverse-phase HPLC, were subjected to automated amino acid sequencing. 5. Over the region sequenced, the salmon GST possessed about 65% homology with the rat and human pi-class GST.

    KW - Amino Acid Sequence

    KW - Animals

    KW - Glutathione Transferase/chemistry

    KW - Humans

    KW - Isoenzymes/chemistry

    KW - Liver/enzymology

    KW - Mammals

    KW - Molecular Sequence Data

    KW - Molecular Weight

    KW - Protein Conformation

    KW - Salmonidae/metabolism

    KW - Sequence Homology, Nucleic Acid

    KW - Species Specificity

    UR - https://www.ncbi.nlm.nih.gov/pubmed/1756623

    M3 - Article

    VL - 100

    SP - 93

    EP - 98

    JO - Comparative Biochemistry and Physiology Part B: Comparative Biochemistry

    JF - Comparative Biochemistry and Physiology Part B: Comparative Biochemistry

    SN - 0305-0491

    IS - 1

    ER -