The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation

Giuseppina Caretti, Louis R. Schiltz, F. Jeffrey Dilworth, Monica Di Padova, Po Zhao, Vasily Ogryzko, Frances V. Fuller-Pace, Eric P. Hoffman, Stephen J. Tapscott, Vittorio Sartorelli

    Research output: Contribution to journalArticle

    174 Citations (Scopus)

    Abstract

    MyoD regulates skeletal myogenesis. Since proteins associated with MyoD exert regulatory functions, their identification is expected to contribute important insights into the mechanisms governing gene expression in skeletal muscle. We have found that the RNA helicases p68/p72 are MyoD-associated proteins and that the noncoding RNA SRA also immunoprecipitates with MyoD. In vitro and in vivo experiments indicated that both p68/p72 and SRA are coactivators of MyoD. RNA interference toward either p68/p72 or SRA prevented proper activation of muscle gene expression and cell differentiation. Unexpectedly, reducing the levels of p68/p72 proteins impaired recruitment of the TATA binding protein TBP; RNA polymerase II; and the catalytic subunit of the ATPase SWI/SNF complex, Brg-1, and hindered chromatin remodeling. These findings reveal that p68/p72 play a critical role in promoting the assembly of proteins required for the formation of the transcription initiation complex and chromatin remodeling.
    Original languageEnglish
    Pages (from-to)547-560
    Number of pages14
    JournalDevelopmental Cell
    Volume11
    Issue number4
    DOIs
    Publication statusPublished - Oct 2006

    Fingerprint

    DEAD-box RNA Helicases
    Untranslated RNA
    Skeletal Muscle
    Chromatin Assembly and Disassembly
    MyoD Protein
    TATA-Box Binding Protein
    Gene Expression
    Proteins
    Muscle Development
    RNA Interference
    Adenosine Triphosphatases
    Cell Differentiation
    Catalytic Domain
    Muscles

    Keywords

    • Cell differentiation physiology
    • Muscle, skeletal physiology
    • MyoD protein metabolism
    • RNA helicases metabolism
    • RNA untranslated metabolism

    Cite this

    Caretti, G., Schiltz, L. R., Dilworth, F. J., Di Padova, M., Zhao, P., Ogryzko, V., ... Sartorelli, V. (2006). The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation. Developmental Cell, 11(4), 547-560. https://doi.org/10.1016/j.devcel.2006.08.003
    Caretti, Giuseppina ; Schiltz, Louis R. ; Dilworth, F. Jeffrey ; Di Padova, Monica ; Zhao, Po ; Ogryzko, Vasily ; Fuller-Pace, Frances V. ; Hoffman, Eric P. ; Tapscott, Stephen J. ; Sartorelli, Vittorio. / The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation. In: Developmental Cell. 2006 ; Vol. 11, No. 4. pp. 547-560.
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    abstract = "MyoD regulates skeletal myogenesis. Since proteins associated with MyoD exert regulatory functions, their identification is expected to contribute important insights into the mechanisms governing gene expression in skeletal muscle. We have found that the RNA helicases p68/p72 are MyoD-associated proteins and that the noncoding RNA SRA also immunoprecipitates with MyoD. In vitro and in vivo experiments indicated that both p68/p72 and SRA are coactivators of MyoD. RNA interference toward either p68/p72 or SRA prevented proper activation of muscle gene expression and cell differentiation. Unexpectedly, reducing the levels of p68/p72 proteins impaired recruitment of the TATA binding protein TBP; RNA polymerase II; and the catalytic subunit of the ATPase SWI/SNF complex, Brg-1, and hindered chromatin remodeling. These findings reveal that p68/p72 play a critical role in promoting the assembly of proteins required for the formation of the transcription initiation complex and chromatin remodeling.",
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    author = "Giuseppina Caretti and Schiltz, {Louis R.} and Dilworth, {F. Jeffrey} and {Di Padova}, Monica and Po Zhao and Vasily Ogryzko and Fuller-Pace, {Frances V.} and Hoffman, {Eric P.} and Tapscott, {Stephen J.} and Vittorio Sartorelli",
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    Caretti, G, Schiltz, LR, Dilworth, FJ, Di Padova, M, Zhao, P, Ogryzko, V, Fuller-Pace, FV, Hoffman, EP, Tapscott, SJ & Sartorelli, V 2006, 'The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation', Developmental Cell, vol. 11, no. 4, pp. 547-560. https://doi.org/10.1016/j.devcel.2006.08.003

    The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation. / Caretti, Giuseppina; Schiltz, Louis R.; Dilworth, F. Jeffrey; Di Padova, Monica; Zhao, Po; Ogryzko, Vasily; Fuller-Pace, Frances V.; Hoffman, Eric P.; Tapscott, Stephen J.; Sartorelli, Vittorio.

    In: Developmental Cell, Vol. 11, No. 4, 10.2006, p. 547-560.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiation

    AU - Caretti, Giuseppina

    AU - Schiltz, Louis R.

    AU - Dilworth, F. Jeffrey

    AU - Di Padova, Monica

    AU - Zhao, Po

    AU - Ogryzko, Vasily

    AU - Fuller-Pace, Frances V.

    AU - Hoffman, Eric P.

    AU - Tapscott, Stephen J.

    AU - Sartorelli, Vittorio

    N1 - dc.publisher: Elsevier (Cell Press) dc.description.sponsorship: Intramural Research Program of the National Institute of Arthritis, Musculoskeletal, and Skin Diseases of the National Institutes of Health

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    N2 - MyoD regulates skeletal myogenesis. Since proteins associated with MyoD exert regulatory functions, their identification is expected to contribute important insights into the mechanisms governing gene expression in skeletal muscle. We have found that the RNA helicases p68/p72 are MyoD-associated proteins and that the noncoding RNA SRA also immunoprecipitates with MyoD. In vitro and in vivo experiments indicated that both p68/p72 and SRA are coactivators of MyoD. RNA interference toward either p68/p72 or SRA prevented proper activation of muscle gene expression and cell differentiation. Unexpectedly, reducing the levels of p68/p72 proteins impaired recruitment of the TATA binding protein TBP; RNA polymerase II; and the catalytic subunit of the ATPase SWI/SNF complex, Brg-1, and hindered chromatin remodeling. These findings reveal that p68/p72 play a critical role in promoting the assembly of proteins required for the formation of the transcription initiation complex and chromatin remodeling.

    AB - MyoD regulates skeletal myogenesis. Since proteins associated with MyoD exert regulatory functions, their identification is expected to contribute important insights into the mechanisms governing gene expression in skeletal muscle. We have found that the RNA helicases p68/p72 are MyoD-associated proteins and that the noncoding RNA SRA also immunoprecipitates with MyoD. In vitro and in vivo experiments indicated that both p68/p72 and SRA are coactivators of MyoD. RNA interference toward either p68/p72 or SRA prevented proper activation of muscle gene expression and cell differentiation. Unexpectedly, reducing the levels of p68/p72 proteins impaired recruitment of the TATA binding protein TBP; RNA polymerase II; and the catalytic subunit of the ATPase SWI/SNF complex, Brg-1, and hindered chromatin remodeling. These findings reveal that p68/p72 play a critical role in promoting the assembly of proteins required for the formation of the transcription initiation complex and chromatin remodeling.

    KW - Cell differentiation physiology

    KW - Muscle, skeletal physiology

    KW - MyoD protein metabolism

    KW - RNA helicases metabolism

    KW - RNA untranslated metabolism

    U2 - 10.1016/j.devcel.2006.08.003

    DO - 10.1016/j.devcel.2006.08.003

    M3 - Article

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    VL - 11

    SP - 547

    EP - 560

    JO - Developmental Cell

    JF - Developmental Cell

    SN - 1534-5807

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