Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts

Judit Pampalona, Jens Januschke, Paula Sampaio, Cayetano Gonzalez

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Drosophila larval neuroblasts (NBs) are an excellent model for asymmetric division and cell cycle studies in general. For decades, visualizing relevant structures like centrosomes, chromosomes, or the mitotic spindle relied exclusively on immunofluorescence on fix samples. More recently, improvements on sensitivity and acquisition speed of different confocal systems have made it possible to acquire time-resolved images of combined fluorescent reporters from single larval NBs. Here, we provide protocols to visualize centrosomes and other organelles from both primary cultures of isolated single NBs and ex vivo, whole-mounted larval brains.

Original languageEnglish
Pages (from-to)301-315
Number of pages15
JournalMethods in Cell Biology
Volume129
DOIs
Publication statusPublished - 2015

Fingerprint

Centrosome
Organelles
Drosophila
Asymmetric Cell Division
Spindle Apparatus
Fluorescent Antibody Technique
Cell Cycle
Chromosomes
Brain

Keywords

  • Cell culture
  • Centriole
  • Centrosome
  • Drosophila
  • Microscopy
  • Neuroblast

Cite this

Pampalona, Judit ; Januschke, Jens ; Sampaio, Paula ; Gonzalez, Cayetano. / Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts. In: Methods in Cell Biology. 2015 ; Vol. 129. pp. 301-315.
@article{091a4c76ed3241e19dca6fba806b2182,
title = "Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts",
abstract = "Drosophila larval neuroblasts (NBs) are an excellent model for asymmetric division and cell cycle studies in general. For decades, visualizing relevant structures like centrosomes, chromosomes, or the mitotic spindle relied exclusively on immunofluorescence on fix samples. More recently, improvements on sensitivity and acquisition speed of different confocal systems have made it possible to acquire time-resolved images of combined fluorescent reporters from single larval NBs. Here, we provide protocols to visualize centrosomes and other organelles from both primary cultures of isolated single NBs and ex vivo, whole-mounted larval brains.",
keywords = "Cell culture, Centriole, Centrosome, Drosophila, Microscopy, Neuroblast",
author = "Judit Pampalona and Jens Januschke and Paula Sampaio and Cayetano Gonzalez",
year = "2015",
doi = "10.1016/bs.mcb.2015.03.003",
language = "English",
volume = "129",
pages = "301--315",
journal = "Methods in Cell Biology",
issn = "0091-679X",
publisher = "Elsevier",

}

Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts. / Pampalona, Judit; Januschke, Jens; Sampaio, Paula; Gonzalez, Cayetano.

In: Methods in Cell Biology, Vol. 129, 2015, p. 301-315.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts

AU - Pampalona, Judit

AU - Januschke, Jens

AU - Sampaio, Paula

AU - Gonzalez, Cayetano

PY - 2015

Y1 - 2015

N2 - Drosophila larval neuroblasts (NBs) are an excellent model for asymmetric division and cell cycle studies in general. For decades, visualizing relevant structures like centrosomes, chromosomes, or the mitotic spindle relied exclusively on immunofluorescence on fix samples. More recently, improvements on sensitivity and acquisition speed of different confocal systems have made it possible to acquire time-resolved images of combined fluorescent reporters from single larval NBs. Here, we provide protocols to visualize centrosomes and other organelles from both primary cultures of isolated single NBs and ex vivo, whole-mounted larval brains.

AB - Drosophila larval neuroblasts (NBs) are an excellent model for asymmetric division and cell cycle studies in general. For decades, visualizing relevant structures like centrosomes, chromosomes, or the mitotic spindle relied exclusively on immunofluorescence on fix samples. More recently, improvements on sensitivity and acquisition speed of different confocal systems have made it possible to acquire time-resolved images of combined fluorescent reporters from single larval NBs. Here, we provide protocols to visualize centrosomes and other organelles from both primary cultures of isolated single NBs and ex vivo, whole-mounted larval brains.

KW - Cell culture

KW - Centriole

KW - Centrosome

KW - Drosophila

KW - Microscopy

KW - Neuroblast

UR - http://www.scopus.com/inward/record.url?scp=84929832565&partnerID=8YFLogxK

U2 - 10.1016/bs.mcb.2015.03.003

DO - 10.1016/bs.mcb.2015.03.003

M3 - Article

C2 - 26175445

VL - 129

SP - 301

EP - 315

JO - Methods in Cell Biology

JF - Methods in Cell Biology

SN - 0091-679X

ER -